Metal enhanced fluorescence from metallic nanoburger structures

• US 8,722,428 B2
• Filed: 11/24/2010
• Issued: 05/13/2014
• Est. Priority Date: 11/25/2009
• Status: Active Grant

• Current Assignee: University of Maryland Baltimore County (University of Maryland)
• Original Assignee: University of Maryland Baltimore County (University of Maryland)
• Inventors: Geddes, Chris D.
• Primary Examiner(s): Haq, Shafiqul
• Assistant Examiner(s): Hollinden, Gary E
• Application Number:
  US13/511,718
• Publication Number:
  US 20130020503A1
• Time in Patent Office: 1,266 Days
• Field of Search: None
• US Class Current: 436/525
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: G01N 21/554 detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 33/54346 Nanoparticles
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/553 Metal or metal coated
• CPC Class Codes: Y10T 428/256 Heavy metal or aluminum or compound thereof

Abstract

The present invention provides for metallic nanostructures or nanoburgers comprising a dielectric layer positioned between metallic layers and their use in metal enhanced emissions systems to enhance emissions from fluorophores, including intrinsic and extrinsic; luminophores; bioluminescent species and/or chemiluminescent species. The multilayer nanoburgers exhibit several distinctive properties including significantly enhanced intensity of emissions, decreased lifetime and increased photostability by simply varying the thickness of the dielectric layer while maintaining a constant thickness of the two metallic layers on opposite sides of the dielectric layer.

Claims

1. A detection system, the system comprising:

a substrate, wherein the substrate is fabricated of at least one material selected from the group consisting of glass, a cellulosic material and polymeric material;

a first and second metalized layer of a multiplicity of metallic structures wherein the first metalized layer is positioned on the substrate, wherein the the first and second metalized layers are separated with a metal oxide layer positioned therebetween, wherein the metal oxide layer has a thickness from about 5 nm to 10 nm and wherein the metallic structures have a geometric shape selected from a triangle, square, sphere, oblong, elliptical, or rectangle,;

at least one excitable molecule that is positioned near at least one of the metalized layers of the metallic structures in a range from about 5 nm to 30 nm;

a source of electromagnetic energy for providing excitation energy to excite the molecule; and

a detector for detecting emissions from the excited molecule and/or the metallic structures.

2. The detection system of claim 1, wherein the first and second metalized layers are fabricated from silver, gold, platinum, aluminum, copper, zinc, chromium, nickel, tin, iron, palladium or composites thereof.

3. The detection system of claim 2 wherein the first and second metalized layers are fabricated from two distinctly different metals.

4. The detection system of claim 1, wherein the metal oxide layer is selected from the group consisting of SiO₂, TiO₂, Fe₂O₃, CuO, ZnO, Y₂O₃, ZrO₂, Nb₂O₅, MoO₃, In₂O₃, SnO₂, Sb₂O₅, Ta₂O₅, WO₃, PbO, Al₂O₃, and now abandoned Bi₂O₃.

5. The detection system of claim 1, wherein the excitable molecule is selected from the group of an intrinsic fluorophore, extrinsic fluorophore, fluorescent dye, and luminophore.

6. A method of metal-enhanced fluorescence sensing, comprising:

providing a substrate, wherein the substrate is fabricated of at least one material selected from the group consisting of glass, a cellulosic material and polymeric material;

providing a first and second metalized layer of metallic structures;

wherein the first metalized layer is positioned on the substrate;

providing a metal oxide layer positioned between the first and second metalized layer of metallic structures, wherein the metal oxide layer has a thickness from about 5 nm to 10 nm;

positioning at least one excitable molecule near at least one of the metalized layers of the metallic structures in a range from about 5 nm to 30 nm;

providing a source of electromagnetic energy for providing excitation energy to excite the molecule; and

detecting emissions from the excited molecule and/or the metallic structures.

7. The method of claim 6, wherein the first and second metalized layers are fabricated from silver, gold, platinum, aluminum, copper, zinc, chromium, nickel, tin, iron, palladium or composites thereof.

8. The method of claim 7, wherein the wherein the first and second metalized layers are fabricated from two distinctly different metals.

9. The method of claim 6, wherein the metal oxide layer is selected from the group consisting of SiO₂, TiO₂, Fe₂O₃, CuO, ZnO, Y₂O₃, ZrO₂, Nb₂O₅, MoO₃, In₂O₃, SnO₂, Sb₂O₅, Ta₂O₅, WO₃, PbO, Al₂O₃, and Bi₂O₃.

10. The method of claim 6, wherein the excitable molecule is selected from the group of an intrinsic fluorophore, extrinsic fluorophore, fluorescent dye, and luminophore.