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Refolding transforming growth factor beta family proteins

  • US 8,722,862 B2
  • Filed: 08/18/2005
  • Issued: 05/13/2014
  • Est. Priority Date: 08/19/2004
  • Status: Expired due to Fees
First Claim
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1. A composition comprising an amount of a refolding buffer effective to, when diluted by a factor of 1 to 10, induce folding of a neublastin polypeptide, wherein the refolding buffer comprises the following components at 1 to 10 times the stated concentrations:

  • (i) potassium phosphate or sodium phosphate at a concentration of 25 mM to 150 mM with a pH ranging from 5.8 to 8.0;

    (ii) guanidine-HCl at a concentration of 0.3 M to 2 M;

    (iii) L-Arginine at a concentration of 0.25 M to 1 M;

    (iv) Tween-80 at a concentration of 0.05% to 1%; and

    (v) oxidized glutathione at a concentration of 1 mM to 4 mM and reduced glutathione at a concentration of 0.05 mM to 0.8 mM, wherein the ratio of oxidized to reduced glutathione is from 5;

    1 to 20;

    1.

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