Parthenolide free bioactive ingredients from feverfew ( Tanacetum parthenium ) and processes for their production
First Claim
1. A bioactive ingredient having anti-inflammatory activity comprising a cytoplasm fraction precipitate derived from cell juice of fresh biomass of a feverfew (Tanacetum parthenium) plant, wherein said cytoplasm fraction precipitate is produced by the process comprising:
- providing fresh biomass of a feverfew (Tanacetum parthenium) plant;
processing the fresh biomass under conditions effective to yield a cell juice supernatant and a membrane fraction;
treating the cell juice supernatant under conditions effective to yield a first cell juice serum supernatant and a cytoplasm fraction precipitate; and
isolating the cytoplasm fraction precipitate, wherein said cytoplasm fraction precipitate is a bioactive fraction that is substantially free of α
-unsaturated γ
-lactones, said α
-unsaturated γ
-lactones comprising parthenolide.
6 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to bioactive ingredients that include isolated bioactive fractions derived from cell juice of fresh biomass of a feverfew (Tanacetum parthenium) plant. The bioactive fractions are either free of or substantially free of α-unsaturated γ-lactones (e.g., parthenolide). Further, the bioactive fractions have anti-inflammatory and antioxidant activity. The present invention also relates to a method for isolating a bioactive fraction that is derived from cell juice of fresh biomass of a feverfew (Tanacetum parthenium) plant and that is at least substantially free of α-unsaturated γ-lactones (e.g., parthenolide). The present invention also relates to a method for preparing a stabilized cell juice serum fraction and a stabilized concentrated cell juice serum fraction that are free of α-unsaturated γ-lactones (e.g., parthenolide). The present invention also relates to a bioactive composition that includes a mixture of one or more of the isolated bioactive fractions of the present invention.
-
Citations
7 Claims
-
1. A bioactive ingredient having anti-inflammatory activity comprising a cytoplasm fraction precipitate derived from cell juice of fresh biomass of a feverfew (Tanacetum parthenium) plant, wherein said cytoplasm fraction precipitate is produced by the process comprising:
-
providing fresh biomass of a feverfew (Tanacetum parthenium) plant; processing the fresh biomass under conditions effective to yield a cell juice supernatant and a membrane fraction; treating the cell juice supernatant under conditions effective to yield a first cell juice serum supernatant and a cytoplasm fraction precipitate; and isolating the cytoplasm fraction precipitate, wherein said cytoplasm fraction precipitate is a bioactive fraction that is substantially free of α
-unsaturated γ
-lactones, said α
-unsaturated γ
-lactones comprising parthenolide.
-
-
2. A bioactive ingredient having anti-inflammatory activity comprising a stabilized cell juice serum fraction derived from cell juice of fresh biomass of a feverfew (Tanacetum parthenium) plant, wherein said stabilized cell juice serum fraction is produced by a process comprising:
-
providing fresh biomass of a feverfew (Tanacetum parthenium) plant; processing the fresh biomass under conditions effective to yield a cell juice supernatant and a membrane fraction; treating the cell juice supernatant under conditions effective to yield a first cell juice serum supernatant and a cytoplasm fraction precipitate; clarifying the first cell juice serum supernatant under conditions effective to yield a second cell juice serum supernatant; and mixing a stabilizing agent with the second cell juice serum supernatant under conditions effective to yield a stabilized cell juice serum fraction, wherein said stabilized cell juice serum fraction is a bioactive fraction that is substantially free of α
-unsaturated γ
-lactones, said α
-unsaturated γ
-lactones comprising parthenolide. - View Dependent Claims (3, 4)
-
-
5. A bioactive ingredient having anti-inflammatory activity comprising a stabilized concentrated cell juice serum fraction derived from cell juice of fresh biomass of a feverfew (Tanacetum parthenium) plant, wherein said stabilized concentrated cell juice serum fraction is produced by a process comprising:
-
providing fresh biomass of a feverfew (Tanacetum parthenium) plant; processing the fresh biomass under conditions effective to yield a cell juice supernatant and a membrane fraction; treating the cell juice supernatant under conditions effective to yield a first cell juice serum supernatant and a cytoplasm fraction precipitate; clarifying the first cell juice serum supernatant under conditions effective to yield a second cell juice serum supernatant; mixing a stabilizing agent with the second cell juice serum supernatant under conditions effective to yield a stabilized cell juice serum fraction; concentrating the stabilized cell juice serum fraction under conditions effective to yield a concentrated cell juice serum supernatant; and mixing a stabilizing agent with the concentrated cell juice serum supernatant under conditions effective to yield a stabilized concentrated cell juice serum fraction, wherein said stabilized concentrated cell juice serum fraction is a bioactive fraction that is free of α
-unsaturated γ
-lactones, said α
-unsaturated γ
-lactones comprising parthenolide. - View Dependent Claims (6, 7)
-
Specification