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Method for sequential sequencing nucleic acids

  • US 8,728,729 B2
  • Filed: 12/04/2012
  • Issued: 05/20/2014
  • Est. Priority Date: 05/23/1997
  • Status: Expired due to Fees
First Claim
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1. A method of sequencing multiple target polynucleotides in parallel using a sequencing system, comprising:

  • providing a flow chamber comprising a plurality of ridges within the flow chamber, the flow chamber comprising multiple microparticles immobilized between the ridges, each microparticle of the multiple microparticles including a target polynucleotide to be sequenced immobilized on the microparticle, the average distance between centers of adjacent microparticles being less than two microparticle diameters, and the ridges being of a height less than a diameter of a microparticle and of a length greater than a plurality of said diameters;

    performing successive cycles of a base-by-base sequencing for the target polynucleotides immobilized on the microparticles in the flow cell; and

    obtaining data that provides sequence information about the plurality of target polynucleotides based on the successive cycles of the base-by-base sequencing.

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