Defined cell culturing surfaces and methods of use
First Claim
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1. A cell culturing substrate comprising:
- a cell culture surface having a film attached thereto, wherein said film comprises one or more plasma polymerized monomers; and
a coating on said film-coated surface, said coating deposited from a coating solution comprising one or more extracellular matrix proteins and an aqueous solvent, wherein the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.
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Abstract
In one aspect, there is provided a cell culturing substrate including:
- a cell culture surface having a film attached thereto, wherein the film includes one or more plasma polymerized monomers; and a coating on the film-coated surface, the coating deposited from a coating solution comprising one or more extracellular matrix proteins and an aqueous solvent, where the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.
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Citations
19 Claims
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1. A cell culturing substrate comprising:
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a cell culture surface having a film attached thereto, wherein said film comprises one or more plasma polymerized monomers; and a coating on said film-coated surface, said coating deposited from a coating solution comprising one or more extracellular matrix proteins and an aqueous solvent, wherein the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification