Detection system for droplet-based assays

• US 8,730,479 B2
• Filed: 12/30/2011
• Issued: 05/20/2014
• Est. Priority Date: 03/25/2010
• Status: Active Grant

• Current Assignee: Bio-Rad Laboratories Incorporated
• Original Assignee: Bio-Rad Laboratories Incorporated
• Inventors: Ness, Kevin D., Arbore, Mark A., Hurst, Jerry E., Klein, David L., Masquelier, Donald A.
• Primary Examiner(s): Pajoohi Gomez, Tara S
• Application Number:
  US13/341,678
• Publication Number:
  US 20120194805A1
• Time in Patent Office: 872 Days
• Field of Search: 3562431-2438, 356244-246, 356436-442
• US Class Current: 356/436
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: G01N 15/1456 without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/052 Tubular type; cavity type; multireflective
• CPC Class Codes: G01N 2021/6419 Excitation at two or more wavelengths
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 2021/8564 Sample as drops
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/31 Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry G01N21/72 takes precedence
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 2201/062 LED's
• CPC Class Codes: G01N 2201/0627 Use of several LED's for spectral resolution
• CPC Class Codes: G01N 2201/0696 Pulsed

Abstract

System, including methods and apparatus, for light detection and signal processing for droplet-based assays. An exemplary system provides a method of detection for droplets. An examination region of a channel may be illuminated with first pulses of light interleaved with second pulses of light as droplets pass through the examination region, the first pulses being spectrally distinct from the second pulses. Data may be collected representing light detected during illumination of the examination region with the first pulses and the second pulses. Each droplet may be illuminated with a beam of light that is narrower than a diameter of the droplets.

Claims

1. A method of detection for droplets, comprising:

illuminating an examination region of a channel with first pulses of light and second pulses of light as droplets of an emulsion pass through the examination region, the first pulses being spectrally distinct from and having a same pulse frequency as the second pulses; and

collecting data representing light detected during illumination of the examination region with the first pulses and the second pulses;

wherein each droplet is illuminated with a beam of light that is narrower than a diameter of the droplets such that no more than a portion of the droplet is illuminated by the beam of light at any given time.

2. The method of claim 1, wherein light for the first pulses is produced by a first light source, and wherein light for the second pulses is produced by a second light source.

3. The method of claim 1, wherein the first pulses, the second pulses, or both the first and second pulses are produced by at least one continuous light beam that is transmitted intermittently to the examination region.

4. The method of claim 1, wherein overlapping volumes of the examination region are illuminated by the first pulses and the second pulses.

5. The method of claim 1, further comprising a step of detecting light from overlapping volumes of the examination region during the first pulses and the second pulses.

6. The method of claim 1, wherein each droplet is illuminated with at least one first pulse and at least one second pulse.

7. The method of claim 1, wherein the data represents light detected with a same detector during illumination of the examination region with the first pulses and the second pulses.

8. The method of claim 1, wherein the step of illuminating includes a step of intersecting the channel with a beam of light having a cross section that is elongated transversely to a long axis defined by the channel.

9. A system for detection in droplet-based assays, comprising:

a channel;

an illumination assembly including a light source and a slit and configured to produce a beam of light that illuminates an examination region of the channel as droplets pass through such region;

a detector configured to detect light received from the examination region; and

a controller that collects data representing light detected by the detector,

wherein light from the light source is shaped by the slit such that the beam of light is elongated in cross section where the beam intersects the channel; and

wherein the beam of light at the examination region is narrower than the diameter of each droplet such that no more than a portion of the droplet is illuminated by the beam of light at any given time.

10. The system of claim 9, wherein the beam of light is elongated in cross section in a direction transverse to a long axis defined by the channel.

11. The system of claim 9, wherein a cross section of the beam, at a position halfway across the channel, extends outside opposing surfaces of the channel.

12. The system of claim 9, wherein the beam of light has a dimension measured parallel to a long axis of the channel where the channel and the beam intersect, and wherein the dimension is less than a diameter of the channel.

13. A method of detection for droplets, comprising:

illuminating an examination region of a channel with first pulses of light and second pulses of light as droplets of an emulsion pass through the examination region, the first pulses being spectrally distinct from and having a same pulse frequency as the second pulses; and

collecting data representing light detected during illumination of the examination region with the first pulses and the second pulses.

14. The method of claim 13, wherein the examination region is illuminated with a second pulse between each successive pair of first pulses and with a first pulse between each successive pair of second pulses.

15. The method of claim 13, wherein a same detector detects light representing the first pulses and the second pulses.

16. The method of claim 13, wherein a first detector detects light representing the first pulses and a second detector detects light representing the second pulses.

17. The method of claim 13, wherein the step of collecting data includes a step of synchronizing generation of first signals with the first pulses and second signals with the second pulses.

18. The method of claim 17, wherein the step of synchronizing includes a step of periodically gating an analog signal from a detector before the analog signal is converted to digital form.

19. The method of claim 17, wherein the first signals represent light detected by a first detector and the second signals represent light detected by a second detector.

20. The method of claim 19, wherein the step of synchronizing includes a step of optically gating light incident on each detector.

21. The method of claim 19, wherein the step of synchronizing includes a step of adjusting a gain of each detector.

22. The method of claim 13, wherein light for the first pulses is produced by a first light source, and wherein light for the second pulses is produced by a second light source.