Humanized neuraminidase antibody and methods of use thereof

• US 8,734,803 B2
• Filed: 09/28/2009
• Issued: 05/27/2014
• Est. Priority Date: 09/28/2008
• Status: Active Grant

• Current Assignee: iBio, Inc.
• Original Assignee: iBio, Inc.
• Inventors: Yusibov, Vidadi, Mett, Vadim, Shoji, Yoko
• Primary Examiner(s): Horning, Michelle S
• Assistant Examiner(s): SALVOZA, M FRANCO G
• Application Number:
  US13/121,235
• Publication Number:
  US 20110311612A1
• Time in Patent Office: 1,702 Days
• Field of Search: None
• US Class Current: 424/147.1
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: A61P 31/16 for influenza or rhinoviruses
• CPC Class Codes: C07K 16/1018 Orthomyxoviridae, e.g. influenza virus
• CPC Class Codes: C07K 16/40 against enzymes
• CPC Class Codes: C07K 2317/24 containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
• CPC Class Codes: C07K 2317/41 Glycosylation, sialylation, or fucosylation
• CPC Class Codes: C07K 2317/56 variable (Fv) region, i.e. VH and/or VL
• CPC Class Codes: C07K 2317/76 Antagonist effect on antigen, e.g. neutralization or inhibition of binding

Abstract

Antibodies against influenza neuraminidase, compositions containing the antibodies, and methods of using the antibodies are provided herein.

Claims

1. An isolated monoclonal antibody that binds neuraminidase, wherein the antibody has the ability to inhibit neuraminidase enzyme activity, and wherein the antibody comprises the light chain variable region amino acid sequence as set forth in amino acids 1 to 127 of SEQ ID NO:

5, and the heavy chain variable region amino acid sequence as set forth in amino acids 1 to 137 of SEQ ID NO;

6.

5. The antibody of claim 1, wherein the antibody is a recombinant antibody.

2. An antigen-binding fragment of an antibody that binds neuraminidase and has the ability to inhibit neuraminidase enzyme activity, wherein the antigen-binding fragment comprises a light chain variable region comprising the amino acid sequence set forth in amino acids 1 to 127 of SEQ ID NO:

5, and a heavy chain variable region comprising the amino acid sequence set forth in amino acids 1 to 137 of SEQ ID NO;

6.

3. The antigen-binding fragment of claim 2, wherein the antigen-binding fragment is an scFv, Fv, Fab′

, Fab, diabody, linear antibody, or F(ab′

)₂ antigen-binding fragment.

4. The antigen-binding fragment of claim 2, wherein the antigen-binding fragment is a complementarity-determining-region (CDR), a univalent fragment, or a single domain antibody.

6. An antibody that binds neuraminidase, wherein the antibody has the ability to inhibit neuraminidase enzyme activity, and wherein the antibody comprises the light chain amino acid sequence set forth in SEQ ID NO:

9 or SEQ ID NO;

10 or the light chain amino acid sequence set forth in SEQ ID NO;

9 or SEQ ID NO;

10 with conservative substitutions such that it is at least 95 percent identical to the amino acid sequence set forth in SEQ ID NO;

9 or SEQ ID NO;

10, and wherein the antibody comprises the heavy chain amino acid sequence set forth in SEQ ID NO;

7 or SEQ ID NO;

8 or the heavy chain amino acid sequence set forth in SEQ ID NO;

7 or SEQ ID NO;

8 with conservative substitutions such that it is at least 95 percent identical to the amino acid sequence set forth in SEQ ID NO;

7 or SEQ ID NO;

8.

7. The antibody of claim 6, wherein the antibody comprises the light chain amino acid sequence set forth in SEQ ID NO:

9 or SEQ ID NO;

10, and the heavy chain amino acid sequence set forth in SEQ ID NO;

7 or SEQ ID NO;

8.

8. The antibody of claim 6, wherein the antibody is produced in a plant.

9. The antibody of claim 6, wherein the antibody is attached to a biological agent or a diagnostic agent.

10. The antibody of claim 9, wherein the antibody is attached to an agent that cleaves a substantially inactive prodrug to release a substantially active drug.

11. The antibody of claim 10, wherein the drug is an anti-influenza agent.

12. The antibody of claim 9, wherein the antibody is attached to an anti-viral agent.

13. The antibody of claim 12, wherein the anti-viral agent is an anti-influenza agent.

14. The antibody of claim 13, wherein the antibody is attached to a diagnostic, imaging or detectable agent.

15. The antibody of claim 14, wherein the antibody is attached to an X-ray detectable compound, a radioactive ion or a nuclear magnetic spin-resonance isotope.

16. The antibody of claim 15, wherein the antibody is attached to:

(a) the X-ray detectable compound bismuth (III), gold (III), lanthanum (III) or lead (II);

(b) the detectable radioactive ion copper⁶⁷, gallium⁶⁷, gallium⁶⁸, indium¹¹¹, indium¹¹³, iodine¹²³, iodine¹²⁵, iodine1³¹, mercury¹⁹⁷, mercury²⁰³, rhenium¹⁸⁶, rhenium¹⁸⁸, rubidium⁹⁷, rubidium¹⁰³, technetium^99m or yttrium⁹⁰;

or (c) the detectable nuclear magnetic spin-resonance isotope cobalt (II), copper (II), chromium (III), dysprosium (III), erbium (III), gadolinium (III), holmium (III), iron (II), iron (III), manganese (II), neodymium (III), nickel (II), samarium (III), terbium (III), vanadium (II) or ytterbium (III).

17. The antibody of claim 14, wherein the antibody is attached to biotin, avidin or to an enzyme that generates a colored product upon contact with a chromogenic substrate.

18. The antibody of claim 9, wherein the antibody is attached to the biological agent as a fusion protein.

19. The antibody of claim 9, wherein the antibody is attached to the biological agent via a biologically releasable bond or selectively cleavable linker.

20. A pharmaceutical composition comprising the antibody of claim 6 and a pharmaceutically acceptable carrier.

21. The composition of claim 20, wherein the composition is formulated for parenteral administration.

22. The composition of claim 20, wherein the antibody is a recombinant, plant-produced antibody.

23. The composition of claim 20, wherein the pharmaceutically acceptable composition is an encapsulated or liposomal formulation.

24. The composition of claim 20, wherein the composition further comprises a second therapeutic agent.