Method for forming a molecularly imprinted polymer biosensor
First Claim
1. A method for forming a molecularly imprinted polymer biosensor, comprising:
- (a) preparing a reaction solution including an imprinting molecule, a functional monomer, an initiator, and a crosslinking agent, wherein a molar ratio of the imprinting molecule, the functional monomer, the initiator and the crosslinking agent with respect to each other in the reaction solution ranges from about 1;
4;
30;
0.17 to 1;
4;
30;
0.85;
(b) disposing the reaction solution in a space between upper and lower substrates each of which is made of a light-transmissible material;
(c) disposing on the upper substrate a photomask having a patterned hole;
(d) irradiating the reaction solution through the patterned hole of the photomask and the upper substrate so that the reaction solution undergoes polymerization to form a polymer between the upper and lower substrates, wherein step (d) is conducted with an irradiation energy ranging from 16 J/cm2 to 72 J/cm2;
(e) removing the upper substrate after the polymer is formed on the lower substrate; and
(f) extracting the imprinting molecule from the polymer so that a patterned molecularly imprinted polymer film is formed on the lower substrate.
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Accused Products
Abstract
A method for forming a molecularly imprinted polymer biosensor includes: (a) preparing a reaction solution including an imprinting molecule, a functional monomer, an initiator, and a crosslinking agent; (b) disposing the reaction solution in a space between upper and lower substrates each of which is made of a light-transmissible material; (c) disposing on the upper substrate a photomask having a patterned hole; (d) irradiating the reaction solution through the patterned hole of the photomask and the upper substrate so that the reaction solution undergoes polymerization to form a polymer between the upper and lower substrates; (e) removing the upper substrate after the polymer is formed on the lower substrate; and (f) extracting the imprinting molecule from the polymer so that a patterned molecularly imprinted polymer film is formed on the lower substrate.
6 Citations
13 Claims
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1. A method for forming a molecularly imprinted polymer biosensor, comprising:
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(a) preparing a reaction solution including an imprinting molecule, a functional monomer, an initiator, and a crosslinking agent, wherein a molar ratio of the imprinting molecule, the functional monomer, the initiator and the crosslinking agent with respect to each other in the reaction solution ranges from about 1;
4;
30;
0.17 to 1;
4;
30;
0.85;(b) disposing the reaction solution in a space between upper and lower substrates each of which is made of a light-transmissible material; (c) disposing on the upper substrate a photomask having a patterned hole; (d) irradiating the reaction solution through the patterned hole of the photomask and the upper substrate so that the reaction solution undergoes polymerization to form a polymer between the upper and lower substrates, wherein step (d) is conducted with an irradiation energy ranging from 16 J/cm2 to 72 J/cm2; (e) removing the upper substrate after the polymer is formed on the lower substrate; and (f) extracting the imprinting molecule from the polymer so that a patterned molecularly imprinted polymer film is formed on the lower substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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Specification