Method to analyze a sample fluid in a microfluidic cytometry system
First Claim
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1. A method of analyzing a sample fluid in a microfluidic cytometry system, the method comprising the steps of:
- a) supplying a sample to a sample well in a microfluidic device;
b) centrifuging the microfluidic device to create a first sample layer and a second sample layer within said sample well;
c) extracting first fluid from said first sample layer; and
d) analyzing said extracted first fluid using cytometry while said extracted first fluid is in the microfluidic device.
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Abstract
The present disclosure relates to microfluidic devices adapted for post-centrifugation use with selective sample extraction, and methods for their use. Certain embodiments make use of a dye-selective sample extraction. Other embodiments make use of a geographically-selective sample extraction. Other embodiments are also disclosed.
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Citations
11 Claims
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1. A method of analyzing a sample fluid in a microfluidic cytometry system, the method comprising the steps of:
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a) supplying a sample to a sample well in a microfluidic device; b) centrifuging the microfluidic device to create a first sample layer and a second sample layer within said sample well; c) extracting first fluid from said first sample layer; and d) analyzing said extracted first fluid using cytometry while said extracted first fluid is in the microfluidic device. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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