Method for making artificial scaffold having porous three-dimensional body comprising cells

• US 8,735,117 B2
• Filed: 09/08/2010
• Issued: 05/27/2014
• Est. Priority Date: 02/25/2005
• Status: Active Grant

• Current Assignee: Drexel University
• Original Assignee: Drexel University
• Inventors: Darling, Andrew, Shor, Lauren, Sun, Wei, Guceri, Selcuk
• Primary Examiner(s): Naff, David M
• Application Number:
  US12/877,818
• Publication Number:
  US 20110165646A1
• Time in Patent Office: 1,357 Days
• Field of Search: None
• US Class Current: 435/177
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36
• CPC Class Codes: A61L 27/46 with phosphorus-containing inorganic fillers
• CPC Class Codes: A61L 27/48 with macromolecular fillers
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: B29C 48/07 Flat, e.g. panels
• CPC Class Codes: B29C 48/11 comprising two or more partially or fully enclosed cavities, e.g. honeycomb-shaped
• CPC Class Codes: B29C 48/12 Articles with an irregular circumference when viewed in cross-section, e.g. window profiles
• CPC Class Codes: C12N 11/02 Enzymes or microbial cells immobilised on or in an organic carrier
• CPC Class Codes: C12N 2533/18 Calcium salts, e.g. apatite, Mineral components from bones, teeth, shells
• CPC Class Codes: C12N 2533/40 Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 2533/54 Collagen; Gelatin
• CPC Class Codes: C12N 2533/56 Fibrin; Thrombin
• CPC Class Codes: C12N 2533/74 Alginate
• CPC Class Codes: C12N 5/0075 using microcarriers

Abstract

In on aspect, the invention includes a microcarrier bead having a porous three-dimensional core having (a) a polymeric porous three-dimensional body having porosity of about 15 to about 90% such that at least 99% of pores are interconnected and have diameters of at most 200 microns, (b) an outer protective layer and optionally (c) a filler. In another aspect, the invention includes a method of making an artificial scaffold wherein a scaffolding material is extruded into a coolant and thereby creating a porous material having a porosity of between 15-90% such that at least 99% of pores are interconnected and have diameters of at most 200 microns.

Claims

1. A method for making an artificial scaffold having a porous three-dimensional body comprising cells and having porosity of about 15 to about 90% such that at least 99% of pores are interconnected and have diameters of at most 200 microns, the method comprising:

providing a scaffolding material;

providing a layer of coolant comprising cells mixed with the coolant, wherein the coolant is at least one of a liquid, a foam, a hydrogel, and wherein the coolant has a temperature of at least 5°

C. lower than a temperature of the scaffolding material, and has a thermal conductivity of greater than 0.026; and

extruding the scaffolding material into the layer of coolant and thereby making the artificial scaffold having a porous three-dimensional body comprising cells wherein at least 99% of pores in the porous three-dimensional body are interconnected and lead to an external surface of the porous three-dimensional body and wherein pores have diameters of at most 200 microns.

2. The method of claim 1, wherein extruding the scaffolding material into the coolant is performed in a layered pattern such that each subsequently extruded layer of the scaffolding material is deposited on top of a previously extruded layer of the scaffolding material.

3. The method of claim 1, wherein the scaffolding material comprises at least one of a biodegradable polymer, fibrin, collagen, and mixtures thereof.

4. The method of claim 3, wherein the scaffolding material further comprises hydroxyapatite.

5. The method of claim 1, further comprising providing a filler to the porous three-dimensional body, wherein the filler is deposited with the scaffolding material.

6. The method of claim 1, further comprising encapsulating the porous three-dimensional body with an outer protective layer.