Fast results hybrid capture assay and system
First Claim
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1. A composition comprising:
- (a) a biological sample suspended in a collection medium, wherein said collection medium comprises NP-40, sodium deoxycholate, and EDTA;
(b) a denaturation reagent;
(c) at least one polynucleotide probe capable of binding to a target nucleic acid molecule;
(d) a support coated with a first antibody; and
(e) a second antibody labeled with a detectable marker.
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Abstract
The present invention comprises a method that provides fast and reliable results for detecting the presence of a target nucleic acid molecule in a sample.
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Citations
24 Claims
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1. A composition comprising:
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(a) a biological sample suspended in a collection medium, wherein said collection medium comprises NP-40, sodium deoxycholate, and EDTA; (b) a denaturation reagent; (c) at least one polynucleotide probe capable of binding to a target nucleic acid molecule; (d) a support coated with a first antibody; and (e) a second antibody labeled with a detectable marker. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A composition comprising:
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(a) a biological sample suspended in a collection medium comprising NP-40, sodium deoxycholate, and EDTA; and (b) a polynucleotide probe. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14)
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15. A kit for the detection of a target nucleic acid molecule in a sample, comprising,
a) a collection medium comprising NP-40, sodium deoxycholate, and EDTA; -
b) a denaturation reagent; c) a support coated with a first anti-hybrid antibody; d) a detection reagent comprising a second anti-poly hybrid antibody, wherein the second antibody is detectably labeled; e) a detergent-based wash buffer; and f) a second detection reagent comprising a substrate for the label on the second antibody. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification