Conductance detection system and method

• US 8,736,287 B2
• Filed: 03/04/2010
• Issued: 05/27/2014
• Est. Priority Date: 03/04/2009
• Status: Active Grant

• Current Assignee: Universal Nanosensor Technologies Inc.
• Original Assignee: Universal Nanosensor Technologies Inc.
• Inventors: Dhirani, Al-Amin, Suganuma, Yoshinori
• Primary Examiner(s): NGUYEN, HOAI AN D
• Application Number:
  US13/255,077
• Publication Number:
  US 20120056632A1
• Time in Patent Office: 1,545 Days
• Field of Search: 324/692, 324/691, 324/649, 324/600, 600/547, 600/300, 600/481, 600/482, 204/228.6, 204/228.1, 204/194, 204/400, 204/403.01, 205/792, 205/775, 205/778.5, 205/779
• US Class Current: 324/692
• CPC Class Codes: G01N 2030/645 electrical conductivity detectors
• CPC Class Codes: G01N 27/4473 by electric means
• CPC Class Codes: G01N 30/6095 Micromachined or nanomachined, e.g. micro- or nanosize
• CPC Class Codes: G01N 30/64 Electrical detectors
• CPC Class Codes: G01N 30/95 Detectors specially adapted therefor; Signal analysis

Abstract

A detection system and method for detecting one or a plurality of species from a mixture of species in a phase. The detection system and method detect changes in conductance induced by the species. The conductance includes a cell structure having a rigid architecture having a top surface a portion of which is electrically conductive forming a first conductive component. A second conductive component substantially overlaps the first conductive component and is spaced from the first conductive component by an insulating component. One or more flow pathways exist between the first conductive component and the second conductive component for the chemical and/or biological species to flow. A time dependent electrical signal for inducing a time dependent response is applied to at least one of said first and second conductive components and a signal detector coupled to at least one of the first and second conductive components measures the time dependent response.

Claims

1. A conductance detector for detecting conductivity and/or dielectric constant of one or more chemical and/or biological species in a phase, comprising:

a cell structure including

a rigid architecture having a top surface a portion of which is electrically conductive forming a first conductive component;

a second conductive component substantially overlapping the first conductive component and spaced from said first conductive component by an insulating component;

one or more flow pathways between the first conductive component and the second conductive component for the chemical and/or biological species to flow;

the first conductive component, the second conductive component and the insulating component having an architecture selected to give

a cross sectional area over which a displacement current and/or external current flows that is on an order of about 1 cm²; and

a distance over which the displacement current and/or external current flows that is in a range from about nanometer to hundreds of microns and even higher;

a power supply for generating a time dependent electrical signal for inducing a time dependent response, the power supply being coupled to at least one of said first and second conductive components;

a signal detector coupled to at least one of said first and second conductive components for measuring the time dependent response; and

a microprocessor connected to said signal detector for determining changes in conductance caused by a presence of said chemical and/or biological species in said phase.

2. The conductance detector according to claim 1 wherein said signal detector is configured to measure one or both of a first component of the first time-dependent response that is an in-phase component related to a change in a sigma conductance and a second component that is an out of phase component related to a change in an epsilon conductance due to the presence of said chemical and/or biological species in said phase.

3. The conductance detector according to claims 1, wherein said power supply includes a generator for generating a plurality of time dependent signals with a plurality of time dependencies, and said power supply is configured to combine the plurality of time-dependent signals and to induce a time-dependent response with a plurality of time dependences.

4. The conductance detector according to claim 1, wherein the rigid architecture comprises a substrate fashioned from any one of silicon, glass, silica, alumina, a printed circuit board, acetate, kapton and plastic.

5. The conductance detector according to claim 1 wherein a flow surface of at least one of said flow pathways of the conductance detector is functionalized with a chemical and/or biological functionality that interacts with said chemical and/or biological species in said phase, wherein said flow surface, the first conductive component and the second conductive component are in close proximity to render significant any change in said sigma conductance and/or said change in said epsilon conductance due to said presence of said chemical and/or biological species.

6. The conductance detector according to claim 1 wherein the cell structure is replaceably housed in a housing or is housed in a microfluidics platform.

7. The conductance detector according to claim 1 wherein the phase being tested for the presence of the chemical and/or biological species is conveyed to the cell structure by a tube or by an adaptor.

8. The conductance detector according to claim 1 wherein the signal detector or the microprocessor are programmed to apply at least one correlation method to improve signal-to-noise of the conductance detector.

9. The conductance detector according to claim 8 wherein the correlation method includes one of Fourier transformation, lock-in techniques, wavelet analysis, Hadamard transforms, Shah convolution Fourier transform analysis or convolution methods.

10. The conductance detector according to claim 1 connected to a chromatography sample output of a chromatography apparatus to detect any chemical and/or biological species present in the chromatography sample output from said chromatography apparatus by said change in said sigma conductance and/or said change in said epsilon conductance caused by the presence of said chemical and/or biological species in said phase.

11. The conductance detector according to claim 10 wherein the chromatography apparatus is a gas chromatography apparatus, a thin layer chromatography apparatus, a high performance liquid chromatography apparatus, an ultrahigh performance liquid chromatography apparatus, or a flash chromatography apparatus.

12. The conductance detector according to claim 1 connected to an electrophoresis sample output of an electrophoresis apparatus to detect said chemical and/or biological species present in the electrophoresis sample output from said electrophoresis apparatus by said change in said sigma conductance and/or said change in said epsilon conductance caused by the presence of said chemical and/or biological species in said phase.

13. The conductance detector according to claim 12 wherein the electrophoresis apparatus is a gel apparatus, a capillary apparatus or a microchannel electrophoresis apparatus.

14. The conductance detector according to claim 1 wherein the cell structure includes a plurality of cavities located in the insulating component into which the phase being tested for the presence of the chemical and/or biological species can enter as the phase flows through the cell structure.

15. The conductance detector according to claim 1 wherein said time dependent response is processed using a time-domain analysis technique including any one or combination of fourier transform, wavelet analysis, differentiation, and high pass filtering to emphasize contributions to said time-dependent response due to said chemical and/or biological species relative to contributions to said time-dependent response due to a changing composition of said phase.

16. The conductance detector according to claim 1 wherein the insulating component is fashioned by spin coating, deposition, or spontaneous oxidation.

17. A conductance detector for detecting conductivity and/or dielectric constant of one or more chemical and/or biological species dispersed in a phase and separated by an integrated substantially planar gel electrophoresis apparatus, comprising:

a first rigid architecture having a top surface a portion of which is electrically conductive forming a first conductive component, the first component being insulated from the phase;

a second conductive component, the second component being insulated from the phase;

a gel component through which displacement current generated by the first or second conductive components flows;

flow pathways through the gel component for the chemical and/or biological species to flow;

a power supply for generating a time dependent electrical signal for inducing a time dependent response, the power supply being coupled to at least one of said first and second conductive components;

a signal detector coupled to at least one of said first and second conductive components for measuring the time dependent response; and

a processor connected to said signal detector configured to determine changes in conductance caused by a presence of said chemical and/or biological species.

18. The conductance detector according to claim 17 wherein the first and second conductive components comprise a plurality of electrode pairs disposed at different locations along the substantially planar gel electrophoresis apparatus such that

the power supply and signal detector are coupled to each of the electrode pairs;

and

said processor and said signal detector configured to determine changes in conductance caused by a presence of said chemical and/or biological species in said phase in a proximity of each electrode pair.

19. The conductance detector according to claim 18 wherein

a first and a second electrode from each pair are disposed on the first rigid architecture and a second rigid architecture, respectively, such that they substantially overlap;

the first and second rigid architecture are spaced by a spacer; and

the gel component, through which the phase containing the chemical and/or biological species to be separated flows, is disposed between the first and second rigid architecture.

20. The conductance detector according to claim 19 wherein the first and second rigid architectures are fashioned from silicon, glass, silica, alumina, a printed circuit board, acetate, kapton, or plastic.

21. The conductance detector according to claim 18, wherein the power supply and signal detector are coupled to each of the electrode pairs using multiplexers.

22. A method for detecting conductivity and/or dielectric constant of one or more chemical and/or biological species in a phase, comprising:

flowing a phase being tested for the one or more chemical and/or biological species through a cell structure, the cell structure including

a rigid architecture having a first electrically conductive component separated from a second conductive component by an insulating component,

one or more flow pathways between the first conductive component and the second conductive component for the chemical and/or biological species to flow,

the first conductive component, the second conductive component and the insulating component having an architecture selected to give

a cross sectional area over which a displacement current and/or external current flows that is on an order of about 1 cm²; and

a distance over which the displacement current and/or external current flows that is in a range from about nanometer to hundreds of microns and even higher;

applying a time dependent electrical signal to at least one of said first and second conductive components for inducing a time dependent response; and

measuring the time dependent response and determining from said time dependent response any changes in conductance caused by a presence of said chemical and/or biological species in said phase.

23. The method according to claim 22 wherein said cell structure comprises said a rigid architecture having a top surface a portion of which is electrically conductive forming said first conductive component, and wherein said second conductive component substantially overlapps the first conductive component and spaced from the first conductive component by the insulating component.

24. The method according to claim 22 wherein said time dependent response is measured by a signal detector is configured to measure one or both of a first component of the first time-dependent response that is an in-phase component related to a change in a sigma conductance and a second component that is an out of phase component related to a change in an epsilon conductance due to the presence of said chemical and/or biological species in said phase.

25. The method according to claim 22 wherein said time dependent electrical signal is applied using a power supply which includes a generator for generating and combining a plurality of time dependent signals with a plurality of time dependencies, and said power supply is configured to combine the plurality of time-dependent signals and to induce a time-dependent response with a plurality of time dependences.

26. The method according to claim 22 wherein the rigid architecture comprises a substrate fashioned from any one of silicon, glass, silica, alumina, a printed circuit board, acetate, kapton and plastic.

27. The method according to claim 22 wherein a flow surface of at least one of said flow pathways of the conductance detector is functionalized with a chemical and/or biological functionality that interacts with said chemical and/or biological species in said phase, wherein said flow surface, the first conductive component and the second conductive component are in close proximity to render significant any change in said sigma conductance and/or said change in said epsilon conductance due to said presence of said chemical and/or biological species.

28. A method for detecting conductivity and/or dielectric constant of one or more chemical and/or biological species dispersed in a phase and separated by an integrated substantially planar gel electrophoresis apparatus, comprising:

flowing said phase containing the one or more chemical and/or biological species through a substantially planar electrophoresis gel component which is located between

a first electrically conductive component, the first electrically component being insulated from the phase and a second electrically conductive component, the second electrically component being insulated from the phase;

a gel component through which displacement current generated by the first or second conductive components flows;

flow pathways through the gel component for the chemical and/or biological species to flow;

applying a time dependent electrical signal for inducing a time dependent response at least one of said first and second conductive components wherein a displacement current is generated by the first or second conductive components flows;

measuring the time dependent response and processing the measured time dependent response for determining changes in conductance caused by a presence of said chemical and/or biological species in said phase.

29. The method according to claim 28 wherein the first and second conductive components comprise a plurality of electrode pairs disposed at different locations along the substantially planar gel electrophoresis apparatus and

a power supply and a signal detector are coupled to each of the electrode pairs;

and

a processor connected to said signal detector configured to determine changes in conductance caused by a presence of said chemical and/or biological species in said phase in a proximity of each electrode pair.

30. The method according to claim 29 wherein

a first and a second electrode from each pair are disposed on the first rigid architecture and a second rigid architecture, respectively, such that they substantially overlap;

the first and second rigid architecture are spaced by a spacer; and

the gel component through which the phase containing the chemical and/or biological species to be separated flows is disposed between the first and second rigid architecture.