Disposable cartridge for fluid analysis

• US 8,741,234 B2
• Filed: 12/27/2011
• Issued: 06/03/2014
• Est. Priority Date: 12/27/2011
• Status: Active Grant

• Current Assignee: Honeywell International Inc.
• Original Assignee: Honeywell International Inc.
• Inventors: Wang, Tzu-Yu, Bardell, Ron, Seifried, Lynn
• Primary Examiner(s): MERKLING, SALLY ANNE
• Application Number:
  US13/337,900
• Publication Number:
  US 20130164854A1
• Time in Patent Office: 889 Days
• Field of Search: 422/417, 422/554
• US Class Current: 422/554
• CPC Class Codes: B01L 2200/0636 Focussing flows, e.g. to laminate flows
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/0874 Three dimensional network
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/058 Flat flow cell
• CPC Class Codes: G01N 2021/513 Cuvettes for scattering measurements
• CPC Class Codes: G01N 2035/00158 Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/51 inside a container, e.g. in an ampoule G01N21/53 takes precedence
• CPC Class Codes: G01N 21/532 with measurement of scattering and transmission
• CPC Class Codes: G01N 2201/0245 with insertable-removable part
• CPC Class Codes: G01N 33/4915 using flow cells flow cytometry G01N15/14

Abstract

A disposable blood analysis cartridge for analyzing a blood sample including an optical absorbance measurement channel is described. The optical absorbance measurement channel includes a plasma separation region and at least one sub channel including a cuvette that is in fluid communication with the plasma separation region and configured to receive a plasma portion of a blood sample that has been passed through the plasma separation region. A negative pressure may be applied to the cartridge to draw the sample through the plasma separation region and into the sub channel including the cuvette.

Claims

1. A disposable blood analysis cartridge for analyzing a blood sample, comprising:

a microfluidics circuit having an optical scattering fluid measurement channel and an optical absorbance measurement channel;

the optical absorbance measurement channel including;

a sample introduction region;

a plasma separation region in fluid communication with the sample introduction region;

a sub channel in fluid communication with the plasma separation region, the sub channel configured to receive a plasma portion of a blood sample that is introduced into the sample introduction region, after the blood sample has passed through the plasma separation region;

the sub channel having a cuvette, and a gas permeable membrane downstream of the cuvette;

a vacuum port situated downstream of the gas permeable membrane; and

wherein a negative pressure applied to the vacuum port may draw the plasma portion of the blood sample from the plasma separation region, into the sub channel and to the cuvette, so that an optical absorbance of the plasma portion of the blood sample can be measured at the cuvette.

2. The disposable blood analysis cartridge of claim 1, wherein:

a plurality of sub channels are in fluid communication with the plasma separation region, wherein each of the plurality of sub channels is configured to receive a plasma portion of a blood sample that is introduced into the sample introduction region, after the blood sample has passed through the plasma separation region;

each of at least two of the sub channels having a corresponding cuvette, and a gas permeable membrane downstream of the corresponding cuvette.

3. The disposable blood analysis cartridge of claim 1, further comprising a waste reservoir for receiving waste fluids produced by the analysis of the blood sample.

4. The disposable blood analysis cartridge of claim 1, wherein at least part of the sample introduction region is configured to mate with and receive at least part of a syringe for delivery of the blood sample into the disposable blood analysis cartridge.

5. The disposable blood analysis cartridge of claim 1, further comprising an additional blood sample introduction region fluidly coupled to the optical scattering fluid measurement channel, wherein the additional blood sample introduction region is separate from the sample introduction region of the optical absorbance measurement channel.

6. The disposable blood analysis cartridge of claim 1, wherein an inner surface of the sample introduction region further comprises an anti-coagulant coating.

7. The disposable blood analysis cartridge of claim 1, wherein the cuvette includes a transparent window.

8. The disposable blood analysis cartridge claim 1, wherein the blood sample introduced into the sample introduction region is a whole blood sample, and is not diluted prior to its introduction into the sample introduction region.

9. The disposable blood analysis cartridge of claim 1, wherein the sample introduction region is a bifurcated sample introduction region having a first sample introduction region in fluid communication with the optical scattering fluid measurement channel, and a second sample introduction region in fluid communication with the optical absorbance measurement channel.

10. The disposable blood analysis cartridge of claim 9, wherein the first sample introduction region and the second sample introduction region are fluidly connected to a common sample introduction port.

11. The disposable blood analysis cartridge of claim 1, wherein at least part of the optical absorbance measurement channel passes over and/or under at least part of the optical scattering fluid measurement channel on the disposable blood analysis cartridge.

12. The disposable blood analysis cartridge of claim 1, further comprising a sample introduction port fluidly coupled to the sample introduction region for introduction of a non-diluted whole blood sample into the sample introduction region of the disposable blood analysis cartridge.

13. The disposable blood analysis cartridge of claim 12, further comprising an addition sample introduction port fluidly coupled to the optical scattering fluid measurement channel.

14. The disposable blood analysis cartridge of claim 12, further comprising a common sample introduction port fluidly coupled to the optical scattering fluid measurement channel and the optical absorbance measurement channel.

15. The disposable blood analysis cartridge of claim 1, further comprising up to five sub-channels in fluid communication with the plasma separation region, each of the sub channels including a cuvette, and a gas permeable membrane located downstream of the cuvette.

16. A method of analyzing a blood sample in a cartridge, the method comprising:

receiving a blood sample in the cartridge, the cartridge having an optical absorbance measurement channel that includes a sample introduction region, a plasma separation region, a sub channel extending from the plasma separation region, the sub channel having a cuvette and a gas permeable membrane downstream of the cuvette, the cartridge also having a vacuum port downstream of the gas permeable membrane;

applying a negative pressure to the vacuum port, such that the negative pressure draws at least some of the blood sample through the plasma separation region, and draws at least some of the plasma from the blood sample from the plasma separation region, into the sub channel, and to the cuvette; and

measuring an optical absorbance of the plasma portion of the blood sample in the cuvette.

17. The method of claim 16, further comprising:

receiving the cartridge in an analyzer that is configured to measure optical absorbance properties of the blood sample;

the analyzer applying the negative pressure to the vacuum port; and

the analyzer measuring the optical absorbance of the plasma portion of the blood sample in the cuvette.

18. The method of claim 17, wherein:

the cartridge further includes an optical scattering fluid measurement channel;

the analyzer is configured to cause at least part of the blood sample to be provided to the optical scattering fluid measurement channel; and

the analyzer is configured to perform an optical scattering measurement on the at least part of the blood sample that is provided to the optical scattering fluid measurement channel.

19. The method of claim 16, wherein the blood sample is an undiluted whole blood sample.

20. A disposable blood analysis cartridge for analyzing a blood sample, comprising:

an optical absorbance measurement channel including;

a sample introduction region;

a plasma separation region in fluid communication with the sample introduction region;

a sub channel in fluid communication with the plasma separation region, the sub channel configured to receive a plasma portion of a blood sample that is introduced into the sample introduction region, after the blood sample has passed through the plasma separation region;

the sub channel having a cuvette, and a gas permeable membrane downstream of the cuvette;

a vacuum port situated downstream of the gas permeable membrane; and

wherein a negative pressure applied to the vacuum port may draw the plasma portion of the blood sample from the plasma separation region, into the sub channel and to the cuvette, so that an optical absorbance of the plasma portion of the blood sample can be measured at the cuvette.

21. The disposable blood analysis cartridge of claim 20, further comprising:

an optical scattering fluid measurement channel, wherein at least part of the optical absorbance measurement channel passes over and/or under at least part of the optical scattering fluid measurement channel on the disposable blood analysis cartridge.