Surface immobilised multilayer structure of vesicles

• US 8,741,577 B2
• Filed: 04/07/2004
• Issued: 06/03/2014
• Est. Priority Date: 04/07/2003
• Status: Expired due to Fees

• Current Assignee: Bio-Rad Laboratories Incorporated
• Original Assignee: Bio-Rad Laboratories Incorporated
• Inventors: Reimhult, Erik, Pfeiffer, Indriati, Granli, Annette, Svedhem, Sofia, Hk, Fredik
• Primary Examiner(s): Haq, Shafiqul
• Assistant Examiner(s): BELEI, CARMENCITA
• Application Number:
  US10/552,649
• Publication Number:
  US 20070116733A1
• Time in Patent Office: 3,709 Days
• Field of Search: 424/1.21, 424/417, 435/6, 435/458, 435/287.2, 436/523, 977/907
• US Class Current: 435/7.1
• CPC Class Codes: G01N 33/5432 Liposomes or microcapsules

Abstract

A surface-immobilized multilayer structure of a plurality of vesicles (2), the structure comprising at least one linker (4) immobilized onto the surface, the at least one linker (4) being bound to at least one other linker, which is attached to a vesicle, which optionally may have another linker (5) bound to another linker (5) attached to another vesicle (2), wherein the structure either comprises at least two vesicles (2) bound via linkers (5) to each other or at least two vesicles (2) bound via linkers (5) attached to the vesicles to one linker (4) immobilized onto the surface (1).

Claims

1. A biologically-functional, surface-immobilized multilayer structure, comprising (i) a support surface, (ii) a plurality of first vesicles formed of a lipid bilayer membrane and spaced apart from said surface, and (iii) a plurality of second vesicles formed of a lipid bilayer membrane and spaced apart from said surface,

wherein the plurality of first vesicles is directly attached to the surface by binding surface-immobilized linkers with vesicle-attached linkers to form a first immobilized layer of the plurality of first vesicles,

wherein the plurality of the second vesicles is attached to the first immobilized layer of the plurality of first vesicles by binding vesicle-attached linkers to vesicle-attached linkers to form two or more immobilized layers of the plurality of second vesicles,

wherein the surface-immobilized linkers and the vesicle-attached linkers comprise oligonucleotides and binding of one linker to another linker is mediated through hybridization of said linker oligonucleotides;

and

wherein at least a selected population of said vesicles comprise a biologically active compound which provides the structure with biological functionality.

2. A structure according to claim 1, wherein said vesicle-attached linkers are attached to said vesicles via at least one of a hydrophobic anchoring moiety comprised in said vesicle-attached linker and a covalent bond to said vesicle via a functionalised group comprised in said vesicle-attached linker.

3. A structure according to claim 1, wherein said vesicles are coated with an outer shell comprising compounds selected from the group consisting of polyethylene glycol, S-layer proteins, peptides, metal clusters and polymers, or wherein vesicle lipids themselves are linked by polymerisation.

4. A structure according to claim 1, wherein the interior volume of said vesicles comprises compounds selected from the group consisting of ions, dyes, drugs, antibodies, enzymes and other proteins.

5. A structure according to claim 1, adapted for release of said multilayer structure from said surface.

6. A structure according to claim 5, designed so that said release is triggered by an electrical potential, light, osmotic stress or incubation with a compound which stimulates said release.

7. A structure according to claim 1, wherein at least two vesicles are attached to each surface-immobilized linker.

8. A structure according to claim 7, wherein said surface-immobilized linker comprises at least one non-linker attached region with a biological functionality.

9. A structure according to claim 8, wherein said non-linker attached region is capable of specific binding with an analyte.

10. A structure according to claim 7, wherein said vesicle-attached linkers are attached to said vesicles via at least one of a hydrophobic anchoring moiety comprised in said linker, and a covalent bond to said vesicle via a functionalised group comprised in said linker.

11. A structure according to claim 7, wherein said vesicles are coated with an outer shell comprising compounds selected from the group consisting of polyethylene glycol, S-layer proteins, peptides, metal clusters and polymers, or wherein vesicle lipids themselves are linked by polymerisation.

12. A structure according to claim 7, wherein the interior volume of said vesicles comprises compounds selected from the group consisting of ions, dyes, drugs, antibodies, enzymes and other proteins.

13. A structure according to claim 7, adapted for release of said multilayer structure from said surface.

14. A structure according to claim 13 designed so that said release is triggered by an electrical potential, light, osmotic stress or incubation with a compound, which stimulates said release.

15. A structure according to claim 1, wherein the vesicles comprise a biologically active compound selected from the group consisting of membrane proteins, antibodies, functionalized lipids, and coupled water-soluble proteins.

16. A structure according to claim 1, wherein the vesicles comprise biologically active membrane proteins.

17. A structure according to claim 1, wherein the vesicles comprise a biologically active compound selected from the group consisting of drugs, proteins, peptides, and oligonucleotides.

18. A method for producing a surface-immobilised multilayer structure according to claim 1, the method comprising the steps of:

(i) providing a support surface comprising linkers immobilised onto the surface, each said surface-immobilised linker being adapted and available for binding to at least one vesicle-attached linker;

(ii) providing vesicles formed of a lipid bilayer membrane, each vesicle comprising outwardly projecting linkers attached thereto, each said vesicle-attached linker being adapted and available for direct binding to a surface-immobilised linker or another vesicle-attached linker, (iii) incubating a first plurality of the vesicles with the surface under conditions promoting binding of vesicle-attached linker(s) directly to the surface-immobilised linker(s) to form a first immobilized layer of the first plurality of vesicles, and incubating at least a second plurality of the vesicles with the surface under conditions promoting binding of vesicle-attached linker(s) directly to the vesicle-attached linker(s) already immobilised, resulting in immobilisation of the second plurality of vesicle(s) and the linker(s) attached thereto to form two or more immobilized layers of the second plurality of vesicles, and (iv) repeating the previous step until the desired amount of vesicles are immobilised,

wherein the surface-immobilized linkers and the vesicle-attached linkers comprise oligonucleotides and binding of one linker to another linker is mediated through hybridization of said linker oligonucleotides, and

wherein at least a selected population of said vesicles comprise a biologically active compound which provides the structure with biological functionality.

19. A method according to claim 18, wherein said surface-immobilised linker comprises at least two sites for binding of vesicle-attached linkers.

20. A method according to claim 18, wherein said surface-immobilised linker comprises only one site for binding of vesicle-attached linkers.

21. A method according to claim 18, wherein said vesicle-attached linkers are attached to said vesicles via at least one of a hydrophobic anchoring moiety comprised in the linker, and a covalent bond to said vesicle via a functionalised group comprised in the linker.

22. A method according to claim 18, wherein said vesicles are coated with an outer shell comprising of compounds selected from the group consisting of polyethylene glycol, S-layer proteins, peptides, metal clusters and polymers.

23. A method according to claim 18, wherein the interior volume of said vesicles comprises compounds selected from the group consisting of ions, dyes, drugs, antibodies, enzymes and other proteins.

24. A method according to claim 18, wherein said surface comprises several surface-immobilised vesicles, which serves as a binding matrix for said structure.

25. A method according to claim 18, wherein said incubation is performed under conditions promoting sequence specific hybridisation of said oligonucleotides.

26. A method according to claim 18, also comprising the step of releasing compounds from the vesicles.

27. A method according to claim 26, wherein said release is triggered by an applied electrical potential osmotic stress or incubation with a compound, which stimulates said release.

28. A method for producing a multilayer structure of a plurality of vesicles, comprising the method according to claim 18, followed by the step of releasing said multilayer structure from said surface.

29. A method according to claim 28, wherein said release is triggered by an electrical potential, osmotic stress or incubation with a compound, which stimulates said release.

30. A biosensor, comprising a structure according to claim 1.

31. A biosensor, comprising a structure produced according to claim 18.

32. The biosensor according to claim 30, wherein said biosensor is an optical biosensor, and said structure is used for increasing the signal of said optical biosensor.

33. The biosensor according to claim 30, wherein said biosensor is a mechanical biosensor, and said structure increases a signal of said mechanical biosensor.