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Amnion-derived cell compositions, methods of making and uses thereof

  • US 8,741,646 B2
  • Filed: 10/06/2011
  • Issued: 06/03/2014
  • Est. Priority Date: 03/31/2005
  • Status: Active Grant
First Claim
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1. A composition comprising conditioned media, wherein the conditioned media is made by a method comprising the steps ofa) obtaining a placenta and isolating an amnion from the placenta,b) enzymatically releasing amnion-derived epithelial cells from the amnion,c) collecting the released amnion-derived epithelial cells,d) culturing the collected amnion-derived epithelial cells of step (c) in basal culture medium that is supplemented with human serum albumin and recombinant human EGF;

  • e) removing the culture medium after about 2-3 days and applying fresh culture medium; and

    f) collecting the culture medium after culturing the cells for 2-3 days.

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