Property effecting and/or property exhibiting compositions for therapeutic and diagnostic uses
First Claim
1. A chemically modified nucleic acid construct, said construct comprising(i) at least one nucleic acid strand consisting of unmodified nucleotides;
- and(ii) one or more nucleic acid strands comprising one or more modified nucleotides or nucleotide analogs, whereinat least one modified nucleotide or nucleotide analog comprises a fusogenic peptide,at least one modified nucleotide or nucleotide analog comprises a ligand to a cell receptor, andat least one modified nucleotide or nucleotide analog comprises a non-nucleic acid entity that confers nuclear localization,and wherein said at least one nucleic acid strand consisting of unmodified nucleotides is a template for the synthesis of a nucleic acid product comprising RNA and having biological activity.
6 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides an array of compositions useful for effecting and/or exhibiting changes in biological functioning and processing within cells and in biological systems containing such cells. In effect, these compositions combine chemical modifications and/or ligand additions with biological functions. The chemical modifications and/or ligand additions provide additional characteristics to the compositions without interfering substantially with their biological function. Such additional characteristics include nuclease resistance, targeting specific cells or specific cell receptors localizing to specific sites within cells and augmenting interactions between the compositions and target cells of interest as well as decreasing such interactions when desired. Also provided by the present invention are processes and kits.
-
Citations
18 Claims
-
1. A chemically modified nucleic acid construct, said construct comprising
(i) at least one nucleic acid strand consisting of unmodified nucleotides; - and
(ii) one or more nucleic acid strands comprising one or more modified nucleotides or nucleotide analogs, wherein at least one modified nucleotide or nucleotide analog comprises a fusogenic peptide, at least one modified nucleotide or nucleotide analog comprises a ligand to a cell receptor, and at least one modified nucleotide or nucleotide analog comprises a non-nucleic acid entity that confers nuclear localization, and wherein said at least one nucleic acid strand consisting of unmodified nucleotides is a template for the synthesis of a nucleic acid product comprising RNA and having biological activity. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 17)
- and
-
11. A chemically modified nucleic acid construct, said construct comprising a modified nucleotide or a nucleotide analog, which construct when present in a cell is used as a template for the synthesis of a nucleic acid product comprising RNA and having biological activity, wherein
said modified nucleotide or nucleotide analog comprises at least one non-nucleic acid entity conferring cell targeting, wherein said construct further comprises at least three strands, wherein the first strand is a template strand comprising a promoter, the second strand (a) has at least one terminus, said terminus comprising a polynucleotide tail and (b) comprises two segments, wherein the first segment is complementary and hybridizes to a portion of the first strand and the second segment lacks said complementarity and comprises said polynucleotide tail, and the third strand comprises (i) said modified nucleotide or nucleotide analog comprising said non-nucleic acid entity conferring cell targeting, and (ii) a polynucleotide sequence hybridized to a complementary sequence in said polynucleotide tail.
Specification