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Preparation of a lipid blend and a phospholipid suspension containing the lipid blend

  • US 8,747,892 B2
  • Filed: 07/25/2013
  • Issued: 06/10/2014
  • Est. Priority Date: 01/14/1998
  • Status: Expired due to Fees
First Claim
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1. A process for preparing a lipid suspension for use with a perfluorocarbon gas as an ultrasound contrast agent, the method comprising:

  • (a) contacting phospholipids with a first non-aqueous solvent which causes the phospholipids to dissolve and form a lipid solution, wherein the contacting comprises the sequential addition of individual phospholipids to the first non-aqueous solvent wherein the phospholipids are 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), 1,2-dipalmitoyl-sn-glycero-3-phosphatidic acid, mono sodium salt (DPPA) and N-(methoxypolyethylene glycol 5000 carbamoyl)-1,2-dipalmitoyl-sn-glycero-3-phosphatidylethanolamine, mono sodium salt (MPEG5000-DPPE), or combining said phospholipids with each other prior to their addition to the first non-aqueous solvent;

    (b) contacting the non-aqueous lipid solution of (a) with a second non-aqueous solvent which causes the phospholipids to precipitate out as a solid lipid blend;

    (c) collecting the solid lipid blend;

    (d) contacting the solid lipid blend with a third non-aqueous solvent which causes the lipid blend to dissolve to form a lipid blend solution;

    (e) contacting the lipid blend solution with an aqueous solution to yield the lipid suspension; and

    (f) filtering the lipid suspension through one or two sterilizing filters to form a filtered lipid suspension.

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