Siliceous particles

• US 8,747,930 B2
• Filed: 06/29/2009
• Issued: 06/10/2014
• Est. Priority Date: 06/29/2009
• Status: Expired due to Fees

• Current Assignee: Aurora Algae, Incorporated (Reliance Industries Limited)
• Original Assignee: Aurora Algae, Incorporated (Reliance Industries Limited)
• Inventors: Fleischer, Daniel, Thompson, Andrew, Jukic, Marko, Radaelli, Guido
• Primary Examiner(s): Riley, Jezia
• Application Number:
  US12/494,170
• Publication Number:
  US 20100330658A1
• Time in Patent Office: 1,807 Days
• Field of Search: 426/425
• US Class Current: 426/425
• CPC Class Codes: C12N 1/12 Unicellular algae; Culture media therefor as new plants A01H13/00
• CPC Class Codes: C12P 7/6463 obtained from glyceride producing microorganisms, e.g. single cell oil

Abstract

Various aspects provide for extracting siliceous particles. Siliceous particles may include or be derived from diatoms. Certain embodiments provide for segregating suspensions into two or more segregation products. In some cases, a first product includes siliceous particles, and a second product may include hydrophobic species. Certain aspects provide for extracting non-siliceous biomass (e.g., lipids).

Claims

1. A method for extracting siliceous particles, the method comprising:

providing a suspension comprising an aqueous liquid and diatoms having siliceous frustules;

adding a nonpolar solvent to the suspension to create a mixture;

agitating the mixture;

segregating the mixture into at least a first product and a second product, the first product having a higher amount of frustules than the second product; and

removing any of the first and second products, wherein the second product includes lipid biomass suitable for production of biofuel, wherein a ratio of the nonpolar solvent to the diatoms is below 1 percent by mass.

2. The method of claim 1, wherein the provided suspension includes a concentration of diatoms in the liquid below 20 weight percent.

3. The method of claim 1, wherein the provided suspension includes a concentration of diatoms in the liquid below 2 weight percent.

4. The method of claim 1, wherein the liquid includes one or more dissolved salts.

5. The method of claim 1, wherein the liquid includes seawater.

6. The method of claim 1, wherein any of the diatoms includes members of the genera Navicula, Amphora, Thalassiosira, Chaetoceros, Nitzschia, Cyclotella, Skeletonema, Phaeodactylum, Achnanthes, Coscinodiscus, Cylindrotheca, Pseudo-Nitzschia, Thalassionema, Hantzschia, Cymbella, Psammodictyon, or any member of the Phylum Bacillariophyta.

7. The method of claim 1, wherein any of the diatoms includes members of the genera Navicula, Amphora, Thalassiosira, Chaetoceros, Nitzschia, Cyclotella, Skeletonema or Phaeodactylum.

8. The method of claim 1, wherein the nonpolar solvent includes an alkane.

9. The method of claim 8, wherein the alkane includes hexane.

10. The method of claim 1, wherein agitating includes ultrasonicating.

11. The method of claim 10, wherein ultrasonicating includes ultrasonicating at an energy density between 10 and 1000 watts per liter of suspension.

12. The method of claim 1, wherein segregating includes accelerating the mixture.

13. A nutrient substance made from siliceous particles fabricated according to a method comprising:

providing a suspension comprising an aqueous liquid and diatoms having siliceous frustules;

adding a nonpolar solvent to the suspension to create a mixture;

agitating the mixture;

segregating the mixture into at least a first product and a second product, the first product having a higher amount of frustules than the second product; and

removing the first product and wherein the second product includes lipid biomass suitable for production of biofuel, wherein a ratio of the nonpolar solvent to the diatoms is below 1 percent by mass.

14. The substance of claim 13, wherein the method further comprises grinding the segregated frustules.

15. A method for providing bioavailable silica to growing organisms, the method comprising:

providing a suspension comprising an aqueous liquid and diatoms having siliceous frustules;

adding a nonpolar solvent to the suspension to create a mixture;

agitating the mixture;

segregating the mixture into at least a first product and a second product, the first product having a higher amount of frustules than the second product;

removing the first product; and

providing the frustules derived from the first product to the growing organisms and wherein the second product includes lipid biomass suitable for production of biofuel, wherein a ratio of the nonpolar solvent to the diatoms is below 1 percent by mass.

16. The method of claim 15, wherein providing includes dissolving the frustules in a liquid in which the growing organisms are growing.

17. A method for extracting siliceous particles, the method comprising:

providing a suspension comprising an aqueous liquid and diatoms having siliceous frustules;

adding a nonpolar solvent to the suspension to create a mixture;

agitating the mixture;

segregating the mixture into at least a first product and a second product, the first product having a higher amount of frustules than the second product; and

removing any of the first and second products, wherein the second product includes lipid biomass suitable for production of biofuel, wherein a ratio of the nonpolar solvent to the diatoms is below 0.1 percent by mass.

18. A nutrient substance made from siliceous particles fabricated according to a method comprising:

providing a suspension comprising an aqueous liquid and diatoms having siliceous frustules;

adding a nonpolar solvent to the suspension to create a mixture;

agitating the mixture;

segregating the mixture into at least a first product and a second product, the first product having a higher amount of frustules than the second product; and

removing the first product and wherein the second product includes lipid biomass suitable for production of biofuel, wherein a ratio of the nonpolar solvent to the diatoms is below 0.1 percent by mass.

19. A method for providing bioavailable silica to growing organisms, the method comprising:

providing a suspension comprising an aqueous liquid and diatoms having siliceous frustules;

adding a nonpolar solvent to the suspension to create a mixture;

agitating the mixture;

segregating the mixture into at least a first product and a second product, the first product having a higher amount of frustules than the second product;

removing the first product; and

providing the frustules derived from the first product to the growing organisms and wherein the second product includes lipid biomass suitable for production of biofuel, wherein a ratio of the nonpolar solvent to the diatoms is below 0.1 percent by mass.