Biodegradation process and composition

• US 8,748,124 B2
• Filed: 12/21/2010
• Issued: 06/10/2014
• Est. Priority Date: 12/23/2009
• Status: Active Grant

• Current Assignee: AMVAC Chemical Corporation (American Vanguard Corporation)
• Original Assignee: Agrinos AS (American Vanguard Corporation)
• Inventors: Rochin, Karl Reiner Fick, Lpez-Cervantes, Jamie, Snchez-Machado, Dalia Isabel
• Primary Examiner(s): Davis, Ruth
• Assistant Examiner(s): Fan, Lynn Y
• Application Number:
  US12/974,924
• Publication Number:
  US 20110151508A1
• Time in Patent Office: 1,267 Days
• Field of Search: 435/42
• US Class Current: 435/42
• CPC Class Codes: C11B 1/025 by enzymes or microorganisms, living or dead
• CPC Class Codes: C12N 1/00 Microorganisms, e.g. protozoa; Compositions thereof medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
• CPC Class Codes: C12N 1/14 Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12R 2001/00 Microorganisms ; Processes using microorganisms

Abstract

Disclosed are novel microbial compositions and biodegradation processes to treat marine animal or marine animal by-products to produce solid, liquid and lipid fractions that contain useful compounds.

Claims

1. A biodegradation process comprising:

mixing a marine animal or marine animal by-product with a microbial composition comprising the microbes in ATCC Patent Deposit Designation PTA-10861 to form a mixture;

fermenting said mixture; and

separating said mixture into solid, aqueous and lipid fractions;

wherein said microbes comprise Bacillus subtilis, Bacillus thuringiensis, Bacillus cereus, Bacillus licheniformis, Bacillus megaterium, Lactobacillus acidophilus, Lactobacillus casei, Pseudomonas fluorescens, Trichoderma harzianum, Rhizobium japonicum, Azotobacter vinelandii, Clostridium pasteurianum, Proteus vulgaris, Streptomyces sp., Nitrobacter sp. and Micrococcus sp.

2. The process of claim 1 wherein said marine animal is a marine arthropod.

3. The process of claim 2 wherein said marine arthropod is selected from the group consisting of shrimp, crab and krill.

4. The process of claim 1 wherein said marine animal is fish.

5. The process of claim 1 wherein said fermenting is by facultative aerobic fermentation.

6. The process of claim 1 wherein said separating is by centrifugation.

7. The process of claim 1 wherein said fermenting is at a temperature of about 30°

C. to 40°

C.

8. The process of claim 7 wherein said fermenting is carried out at a pH between about 4.3 and 5.0.

9. The process of claim 7 wherein said fermentation is carried out for about 24-96 hours.

10. The process of claim 1 wherein said aqueous fraction comprises amino acids, chitosan and glucosamine.

11. The process of claim 10 wherein said aqueous fraction further comprises trace elements.

12. The aqueous fraction made according to claim 1.

13. The process of claim 1 wherein said solid fraction comprises chitin.

14. The solid fraction made according to claim 13.