Culture of cardiovascular cells on a matrix and method for regenerating cardiovascular tissue

• US 8,748,142 B2
• Filed: 11/10/2009
• Issued: 06/10/2014
• Est. Priority Date: 09/09/1999
• Status: Expired due to Fees

• Current Assignee: Gunze Limited, Tokyo Women's Medical University
• Original Assignee: Gunze Limited, Tokyo Women's Medical University
• Inventors: Imai, Yasuharu, Morita, Shinichiro, Shin'Oka, Toshiharu
• Primary Examiner(s): Naff, David M
• Application Number:
  US12/616,000
• Publication Number:
  US 20100055791A1
• Time in Patent Office: 1,673 Days
• US Class Current: 435/180
• CPC Class Codes: A61L 2430/20 for reconstruction of the heart, e.g. heart valves
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/3808 Endothelial cells
• CPC Class Codes: A61L 27/3826 Muscle cells, e.g. smooth muscle cells
• CPC Class Codes: A61L 27/383 Nerve cells, e.g. dendritic cells, Schwann cells
• CPC Class Codes: A61L 27/3839 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3843 Connective tissue
• CPC Class Codes: A61L 27/3886 comprising two or more cell types
• CPC Class Codes: A61L 27/48 with macromolecular fillers
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: A61L 27/58 Materials at least partially resorbable by the body
• CPC Class Codes: C08L 67/04 Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence
• CPC Class Codes: C12N 2502/1323 Adult fibroblasts
• CPC Class Codes: C12N 2533/40 Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/069 Vascular Endothelial cells

Abstract

Materials for culturing cardiovascular tissues wherein a sponge made of a bioabsorbable material is reinforced with a reinforcement made of a bioabsorbable material.

Claims

1. A culture of cardiovascular cells on a matrix to regenerate cardiovascular tissue comprising a sponge made of a bioabsorbable material and a reinforcement,

the sponge comprising lactic acid (D form, L form, DL form)-caprolactone copolymer,

the reinforcement comprising at least one member selected from the group consisting of polyglycolic acid and polylactic acid (D form, L form, DL form),

the reinforcement being integrated with the sponge,

the reinforcement having a greater strength than the sponge,

the cardiovascular tissue being at least one member selected from the group consisting of a blood vessel and a cardiac valve, and

the cardiovascular cells being a mixed cell culture of two or three different kinds selected from the group consisting of endothelial cells, smooth muscle cells and fibroblasts.

2. The culture on the matrix according to claim 1 for use in regenerating an artery, wherein the reinforcement comprises polylactic acid (D form, L form, DL form).

3. The culture on the matrix according to claim 1 for use in regenerating a vein, wherein the reinforcement comprises polyglycolic acid.

4. The culture on the matrix according to claim 1 for use in regenerating a cardiac valve or a pericardium, wherein the reinforcement comprises polylactic acid (D form, L form, DL form).

5. The culture on the matrix according to claim 1, wherein the sponge has a pore diameter of about 5 μ

m to about 100 μ

m.

6. A method for regenerating cardiovascular tissue comprising culturing the cells of the culture on the matrix of claim 1.

7. The method for regenerating cardiovascular tissue according to claim 6, wherein the cardiovascular tissue regenerated is a blood vessel.

8. The method for regenerating cardiovascular tissue according to claim 6, wherein the cardiovascular tissue regenerated is a cardiac valve.

9. The culture on the matrix according to claim 1, wherein the reinforcement integrated with the sponge is obtained by lyophilizing a solution comprising lactic acid (D form, L form, DL form)-caprolactone copolymer in contact with a reinforcement selected from the group consisting of a fiber, a nonwoven cloth and a film.

10. The culture on the matrix according to claim 1, wherein the reinforcement has a shape selected from a fiber, nonwoven cloth and film.