Cell population enriched for endoderm cells

• US 8,748,171 B2
• Filed: 07/14/2010
• Issued: 06/10/2014
• Est. Priority Date: 05/17/2002
• Status: Expired due to Term

• Current Assignee: Icahn School of Medicine at Mount Sinai
• Original Assignee: Mount Sinai School of Medicine (Sinai Health System)
• Inventors: Kouskoff, Valerie, Kubo, Atsushi, Fehling, Hans Joerg, Keller, Gordon M
• Primary Examiner(s): Wilson, Michael C.
• Application Number:
  US12/836,275
• Publication Number:
  US 20120114613A1
• Time in Patent Office: 1,427 Days
• Field of Search: 435/325, 435/455
• US Class Current: 435/325
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 21/04 for myasthenia gravis
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 35/02 specific for leukemia
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 7/06 Antianaemics
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: A61P 9/04 Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
• CPC Class Codes: C12N 2500/90 Serum-free medium, which may still contain naturally-sourced components
• CPC Class Codes: C12N 2501/16 Activin; Inhibin; Mullerian inhibiting substance
• CPC Class Codes: C12N 2501/415 Wnt; Frizzeled
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 5/0603 Embryonic cells production of embryos, nuclear transfer A01K67/027; Embryoid bodies
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696
• CPC Class Codes: G01N 33/5073 Stem cells

Abstract

The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. The cell populations of the invention are useful for generating cells for cell replacement therapy.

Claims

1. An isolated cell population enriched for endoderm cells derived from mammalian embryoid bodies (EB) cultured in activin at a concentration of about 30 to about 100 ng/ml of activin, wherein the cell population is enriched for endoderm cells that express Sox-17, Hex-1, and HNF3β

and do not express GATA1 or PAX6.

2. The cell population of claim 1 wherein the concentration of activin is about 100 ng/ml and about 50% of the cells in the cell population express HNF3β

and Hex-1.

3. The cell population of claim 1, wherein the endoderm cells that express Sox-17, Hex-1, and HNF3β

and do not express GATA1 or PAX6 are capable of expressing albumin (ALB), α

-fetoprotein (AFP), and surfactant protein C (SP-C).