Biofilm-removing antimicrobial compositions and uses thereof

• US 8,753,692 B2
• Filed: 01/22/2010
• Issued: 06/17/2014
• Est. Priority Date: 01/23/2009
• Status: Active Grant

• Current Assignee: Kane Biotech Inc.
• Original Assignee: Kane Biotech Inc.
• Inventors: Gawande, Purushottam V., Madhyastha, Srinivasa, Lovetri, Karen, Yakandawala, Nandadeva, Froehlich, Gord
• Primary Examiner(s): Haghighatian, Mina
• Assistant Examiner(s): HIRT, ERIN E
• Application Number:
  US13/145,991
• Publication Number:
  US 20110311647A1
• Time in Patent Office: 1,607 Days
• Field of Search: None
• US Class Current: 424/618
• CPC Class Codes: A01N 2300/00 Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
• CPC Class Codes: A01N 25/34 Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group
• CPC Class Codes: A01N 33/12 Quaternary ammonium compounds
• CPC Class Codes: A01N 43/54 1,3-Diazines; Hydrogenated 1,3-diazines
• CPC Class Codes: A01N 47/44 Guanidine; Derivatives thereof
• CPC Class Codes: A01N 59/12 Iodine, e.g. iodophors; Compounds thereof
• CPC Class Codes: A01N 59/16 Heavy metals; Compounds thereof
• CPC Class Codes: A01N 63/10 Animals; Substances produced thereby or obtained therefrom
• CPC Class Codes: A01N 63/50 Isolated enzymes; Isolated proteins peptides A01N37/46
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 31/09 having two or more such linkages
• CPC Class Codes: A61K 31/14 Quaternary ammonium compounds, e.g. edrophonium, choline betaines A61K31/205
• CPC Class Codes: A61K 31/155 Amidines (), e.g. guanidine (H2N—C(=NH)—NH2), isourea (N=C(OH)—NH2), isothiourea (—N=C(SH)—NH2)
• CPC Class Codes: A61K 31/513 having oxo groups directly attached to the heterocyclic ring, e.g. cytosine
• CPC Class Codes: A61K 31/635 having a heterocyclic ring, e.g. sulfadiazine
• CPC Class Codes: A61K 33/38 Silver; Compounds thereof
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61K 8/20 Halogens; Compounds thereof
• CPC Class Codes: A61K 8/416 Quaternary ammonium compounds A61K8/35 takes precedence
• CPC Class Codes: A61K 8/43 Guanidines
• CPC Class Codes: A61K 8/4953 : containing pyrimidine ring derivatives, e.g. minoxidil
• CPC Class Codes: A61K 8/64 : Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61L 12/088 : Heavy metals
• CPC Class Codes: A61P 31/00 : Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 31/02 : Local antiseptics
• CPC Class Codes: A61P 31/04 : Antibacterial agents
• CPC Class Codes: A61P 31/10 : Antimycotics
• CPC Class Codes: A61P 43/00 : Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61Q 17/005 : Antimicrobial preparations
• CPC Class Codes: Y02A 50/30 : Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention provides novel compositions comprising: (a) protamine sulfate and (b) benzalkonium chloride or a silver containing particle; or (a) sodium metaperiodate and (b) 5-fluorouracil or chlorhexidine and uses thereof for the preparation of devices, and in particular medical devices, susceptible to colonization by biofilm forming bacteria.

Claims

1. A composition for removing or reducing microbial biofilms, wherein the biofilm is caused by one or more of Pseudomonas aeruginosa, Staphylococcus epidermis, and Escherichia coli, comprising:

(a) a sodium meta-periodate and (b) chlorhexidine.

2. The composition according to claim 1, wherein the concentration of sodium meta-periodate comprises about 20 μ

g/mL to about 2000 μ

g/mL sodium meta-periodate.

3. The composition according to claim 1, wherein the chlorhexidine base or salts comprises about 1 μ

g/mL to about 100 μ

g/mL chlorhexidine.

4. A method to reduce or remove microbial biofilms, wherein the microbial biofilm is caused by one or more of Pseudomonas aeruginosa, Staphylococcus epidermis, and Escherichia coli comprising disinfecting, cleaning, or rinsing a surface by contacting said surface with a combination of a composition of claim 1 and a disinfectant.

5. The composition according to claim 1, wherein said composition is incorporated in a toothbrush;

dental floss;

a denture, a mouth guard;

a dairy line;

a dairy line filter;

a water line;

a line used in food and beverage manufacturing;

a general household disinfectant;

a laundry detergent;

cleaning supplies;

equipment involved in the leeching process or mining;

wound care;

a vacuum system;

an HVAC system;

a vacuum cleaner bag;

paint covering;

a wall covering;

a window frame;

a door;

a door frame;

a cooling tower;

a humidifier;

a vacuum cleaner;

a filter;

a toy;

a cosmetic container;

a plastic bottle;

a water jug;

a tap and water spout;

a washing machine;

a dishwasher;

an animal water dish;

a bathroom tile;

a bathroom fixture;

a sink;

a shower;

a shower head;

a toilet;

a toilet lid;

a toilet seat;

a sealant;

grout;

a towel;

a TUPPERWARE®

container;

a dish;

a cup;

an utensil;

a bowl;

a food storage container;

a beverage storage container;

a cutting board;

a dish drying tray;

a garbage bag;

a bathtub;

a whirlpool;

a Jacuzzi bathtub;

a sink;

a shower;

a fish pond;

a swimming pool;

a swimming pool liner;

a swimming pool skimmer;

a pond liner;

a bird bath;

a garden hose;

a water sprinkling line;

a planter; and

a hot tub.

6. A method of disinfecting a wound comprising administering a composition according to claim 1 to the wound.

7. A method of spraying, dipping, overlaying, or coating an object to be disinfected comprising contacting said object with a composition according to claim 1.

8. The method as claimed in claim 7 wherein the object is selected from the group consisting of:

a denture;

a mouth guard;

a dairy line;

a water line;

an adhesive bandage;

a component of an HVAC system;

a component of a water treatment facility;

a component of a vacuum or a vacuum cleaner;

a vacuum cleaner bag;

a vacuum cleaner filter;

an air filter;

a component of a cooling tower;

a toy;

a window;

a door;

a window frame;

a door frame;

a medical instrument;

a dental instrument;

a bathroom tile;

a kitchen tile;

food industry processing instruments;

hospital tables and beds;

an animal water dish;

a washing machine;

a dish washer;

a towel;

a dish;

a bowl;

a utensil;

a cup;

a glass;

a cutting board;

a dish drying tray;

a whirlpool bathtub;

a sink;

a toilet;

a toilet seat;

a swimming pool;

a bird bath;

a planter;

a garden hose;

a fish pond;

an oil pipe;

a gas pipe;

a dairy line filter;

a line used in food and beverage manufacturing;

a cosmetic container;

an outdoor pond liner;

a tap and water spout;

a humidifier;

a humidifier filter;

a bathroom tile;

a bathroom fixture;

a toilet lid;

a swimming pool liner;

a swimming pool skimmer;

a swimming pool filter;

a hot tub line;

a hot tub filter;

a washing machine liner;

a dishwasher liner;

an animal water dish;

a food storage container;

a beverage storage container;

a plate;

a cup;

a fork;

a knife;

a spoon;

a garbage bag; and

a countertop.