Assays for resistance to echinocandin-class drugs
First Claim
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1. A nucleic acid assay for detecting echinocandin drug resistance mutations in fungi susceptible to echinocandin drugs and containing the FKS1 gene corresponding to 1-3β
- -D-glucan synthase subunit Fks1p comprising exponentially amplifying a first nucleic acid target sequence of the FKS1 gene corresponding to at least a portion of CaFks1p amino acids 636 to 654 that includes amino acids 641 to 649 to generate an amplified first target sequence, contacting the amplified first target sequence with a first labeled hybridization probe specific for an amino acid mutation selected from the group consisting of S645P, S645Y, and S645F of CaFks1p, and detecting the presence of the mutation, thereby detecting an echinocandin drug resistance mutation.
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Abstract
Nucleic acid amplification assays for mutations to two short sections of the fungal gene FKS1. Mutations in these target sequences have been shown to correlate with resistance to echinocandin-class drugs. Assays may include detection by sequencing or by labeled hybridization probes. Also, primers, probes and reagent kits for performing such assays.
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12 Claims
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1. A nucleic acid assay for detecting echinocandin drug resistance mutations in fungi susceptible to echinocandin drugs and containing the FKS1 gene corresponding to 1-3β
- -D-glucan synthase subunit Fks1p comprising exponentially amplifying a first nucleic acid target sequence of the FKS1 gene corresponding to at least a portion of CaFks1p amino acids 636 to 654 that includes amino acids 641 to 649 to generate an amplified first target sequence, contacting the amplified first target sequence with a first labeled hybridization probe specific for an amino acid mutation selected from the group consisting of S645P, S645Y, and S645F of CaFks1p, and detecting the presence of the mutation, thereby detecting an echinocandin drug resistance mutation.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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