Biochip
First Claim
Patent Images
1. A DNA chip, comprising:
- a flat carrier;
an array of spots containing catcher molecules, each spot being assigned a microelectrode arrangement for detecting binding events between the catcher molecules and target molecules applied via an analyte solution, wherein the microelectrode arrangement is at least a two-pole system,the microelectrode arrangement being at least partially embedded in a hydrophilic reaction layer which is permeable to target molecules and in which immobilized catcher molecules are distributed three-dimensionally, wherein the catcher molecules are configured to cause the binding events,the hydrophilic reaction layer having a thickness approximately in the range of 1L to 5 L, L being the sum of electrode width and electrode spacing, the electrode width and the electrode spacing being approximately 1 μ
m,wherein the chip is configured for application of an alternating voltage in a megahertz frequency, andwherein the chip is configured to detect a capacitance change caused by the binding events within the hydrophilic reaction layer with a megahertz frequency applied to the electrodes.
2 Assignments
0 Petitions
Accused Products
Abstract
A biochip includes a flat carrier and an array of spots containing catcher molecules which are arranged on the carrier. Each spot is associated with a microelectrode arrangement for impedance spectroscopic detection of binding events occurring between the catcher molecules and the target molecules applied via an analyte solution. In order to increase the sensitivity or the binding-specific measuring effects of the bio-chip, the electrode arrangement is at least partially embedded in a hydrophilic reaction layer containing catcher molecules and which is permeable to target molecules.
-
Citations
16 Claims
-
1. A DNA chip, comprising:
-
a flat carrier; an array of spots containing catcher molecules, each spot being assigned a microelectrode arrangement for detecting binding events between the catcher molecules and target molecules applied via an analyte solution, wherein the microelectrode arrangement is at least a two-pole system, the microelectrode arrangement being at least partially embedded in a hydrophilic reaction layer which is permeable to target molecules and in which immobilized catcher molecules are distributed three-dimensionally, wherein the catcher molecules are configured to cause the binding events, the hydrophilic reaction layer having a thickness approximately in the range of 1L to 5 L, L being the sum of electrode width and electrode spacing, the electrode width and the electrode spacing being approximately 1 μ
m,wherein the chip is configured for application of an alternating voltage in a megahertz frequency, and wherein the chip is configured to detect a capacitance change caused by the binding events within the hydrophilic reaction layer with a megahertz frequency applied to the electrodes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
-
Specification