Multi-dimensional fluid sensors and related detectors and methods

• US 8,753,893 B2
• Filed: 06/12/2009
• Issued: 06/17/2014
• Est. Priority Date: 06/19/2008
• Status: Expired due to Fees

• Current Assignee: Ben H. Liu
• Original Assignee: Ben H. Liu
• Inventors: Liu, Ben H., Soohoo, Jeffrey R., Vidt, Meghan E.
• Primary Examiner(s): BROWN, MELANIE YU
• Application Number:
  US12/994,302
• Publication Number:
  US 20110076783A1
• Time in Patent Office: 1,831 Days
• Field of Search: 436/518, 436/524, 436/525, 436/526, 436/149, 435/7.1, 435/283.1, 435/287.1, 435/287.2, 422/50, 422/68.1, 422/82.01
• US Class Current: 436/149
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0663 Whole sensors
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 1/6813 Hybridisation assays
• CPC Class Codes: G01N 15/01 specially adapted for biological cells, e.g. blood cells investigating sedimentation of particle suspensions in blood G01N15/05
• CPC Class Codes: G01N 15/0656 using electric, e.g. electrostatic methods or magnetic methods by investigating individual particles G01N15/1031, G01N15/12
• CPC Class Codes: G01N 27/27 Association of two or more measuring systems or cells, each measuring a different parameter, where the measurement results may be either used independently, the systems or cells being physically associated, or combined to produce a value for a further parameter
• CPC Class Codes: G01N 33/569 for microorganisms, e.g. protozoa, bacteria, viruses

Abstract

The present invention provides multi-dimensional sensors with fluidic flow channels for processing fluid samples.

Claims

1. A multi-dimensional fluidic sensor device, comprising:

a plurality of sensors, each sensor comprising a set of associated electrodes, including at least one working electrode, a reference electrode and a counter electrode, with each electrode in the electrode set positioned one above another and isolated by an electrical insulator therebetween, and wherein each set of electrodes has aligned apertures that extend through each of the electrodes to define at least a part of a fluidic flow channel.

2. A sensor according to claim 1, wherein the aligned apertures include a respective working electrode aperture, a reference electrode aperture and a counter electrode aperture, and wherein the working electrode has an inner wall that surrounds and defines the working electrode aperture, and wherein at least a portion of the inner wall comprises a predetermined material analyte for contacting a sample flowing through a respective fluidic flow channel.

3. A sensor according to claim 2, wherein the predetermined material comprises a bioactive material of one or more of the following:

an antibody, an antigen, a nucleic acid, a peptide nucleic acid, a ligand, a receptor, avidin, biotin, Protein A, Protein G, Protein L, a substrate for an enzyme and any combination thereof.

20. A method of monitoring fluid samples for detecting waterborne or airborne toxins or pathogens, comprising:

providing a sensor device according to claim 1;

flowing fluid samples through fluidic flow channels comprising the sensors; and

electronically detecting when a fluid sample tests positive for a selected analyte based on an output of the at least one sensor in a respective fluidic flow channel.

21. A fluidic monitoring system according to claim 20, wherein the fluidic flow channels comprise a plurality of different sensors configured to test for different pathogens or toxins, and wherein the flowing step comprises serially flowing a respective fluid sample through a plurality of different fluidic flow channels in the sensor body.

4. A multi-dimensional fluidic sensor device, comprising:

a plurality of sensors, each sensor comprising a set of associated electrodes, including at least one working electrode, a reference electrode and a counter electrode, with each electrode in the electrode set positioned one above another and isolated by an electrical insulator therebetween, and wherein each set of electrodes has aligned apertures that define at least a part of a fluidic flow channel,

wherein the multi-dimensional sensor has a top surface and a bottom surface with a plurality of apertures arranged in columns and rows that form a plurality of fluidic flow channels, and wherein at least one set of the electrodes define at least a part of at least some of the plurality of fluidic flow channels.

5. A sensor according to claim 4, wherein the plurality of fluidic flow channels are microfluidic flow channels.

6. A sensor according to claim 4, wherein the fluidic flow channels comprise a plurality of sensors, each sensor comprising at least one working electrode, and wherein each working electrode resides in a respective fluid channel vertically spaced apart from another working electrode.

7. A sensor according to claim 6, wherein at least some of the sensors in at least one fluidic flow channel share a common reference and/or counter electrode.

8. A sensor according to claim 4, wherein the sensor is a 4-D sensor array comprising a plurality of sensors in each fluidic flow channel.

9. A sensor according to claim 8, wherein the 4-D sensor array is defined by a vertically stacked assembly of a plurality of 3-D sensor arrays, each of the 3-D sensor arrays having a single sensor in a respective fluidic flow channel, and wherein the plurality of 3-D sensors comprise at least one fluid seal that resides between adjacent pairs of 3-D sensors and seals the respective fluidic flow channels.

10. A multi-dimensional fluidic sensor device, comprising:

a plurality of sensors, each sensor comprising a set of associated electrodes, including at least one working electrode, a reference electrode and a counter electrode, with each electrode in the electrode set positioned one above another and isolated by an electrical insulator therebetween, and wherein each set of electrodes has aligned apertures that define at least a part of a fluidic flow channel,

wherein each sensor comprises a plurality of spaced apart working electrodes, one residing above another and separated by an electrical insulator.

11. A three-or four dimensional fluidic sensor, comprising:

a sensor body having an array of fluidic flow channels formed therethrough, wherein the fluidic flow channels comprise at least one sensor having;

at least one working electrode having an upwardly extending inner wall surrounding an aperture;

at least one counter electrode having an upwardly extending inner wall surrounding an aperture and residing above or below the at least one working electrode; and

at least one reference electrode having an upwardly extending inner wall surrounding an aperture and residing above or below the at least one counter electrode,

wherein the working electrode aperture, the counter electrode aperture and the reference electrode aperture are aligned to define at least a portion of the fluidic flow channels.

12. A fluidic sensor according to claim 11, further comprising an electrical insulator positioned between each of the electrodes.

13. A fluidic sensor according to claim 11, wherein the sensor body comprises a plurality of stacked layers, including at least one working electrode layer, at least one reference electrode layer, and at least one counter electrode layer, each layer having an array of apertures thereon configured and sized so that, when aligned, the array of apertures of each of the layers defines at least a portion of the respective fluidic flow channels.

14. A fluidic sensor according to claim 13, wherein the layers are sealed together, integral with each other or snugly attached to define discrete fluid-tight fluidic flow channels.

15. A fluidic sensor according to claim 11, wherein the sensor body comprises a plurality of sensors in each fluidic flow channel, each sensor having at least one working electrode that is vertically spaced apart above or below another working electrode of another sensor in a respective fluidic flow channel, and wherein the sensor comprises between about 50 to about 100,000 fluidic flow channels.

16. A fluidic sensor according to claim 15, wherein the respective sensors in a respective fluidic flow channel are configured to detect a different target analyte in a fluid sample flowing therethrough.

17. A fluidic sensor according to claim 11, wherein the working electrode comprises a material on or in the inner wall that causes the working electrode to produce a detectable electrical signal or change in an optical or magnetically detectable manner when a fluid having a predetermined substance therein is or has been in fluid communication with the inner wall of the working electrode in a respective fluidic flow channel.

18. A fluidic sensor according to claim 11, wherein each sensor comprises a plurality of vertically spaced apart working electrodes with respective apertures that define at least a portion of a respective fluidic flow channel.

19. A fluidic sensor according to claim 11, further comprising electrical traces that extend from each sensor to an outer perimeter of the sensor body for engaging an electrical interface circuit that can communicate with the working, reference and counter electrodes of each respective sensor.

22. A fluidic detector system for analyzing fluid samples for target substances or materials, comprising:

a sensor body having a plurality of fluid flow channels extending therethrough, the flow channels comprising a plurality of sensors, each sensor having at least one upwardly extending working electrode with a working electrode aperture, at least one counter electrode with a counter electrode aperture, and at least one reference electrode with a reference electrode aperture, wherein the apertures are aligned to define a portion of a respective fluid flow channel, and wherein at least one of the sensors is configured to test for a different selected analyte in a fluid sample relative to at least one other sensor; and

a sensor detector in communication with the sensor body, the sensor detector configured to electronically poll each sensor in each flow channel to obtain a signal associated with a positive or negative test in response to the fluid sample passing through the fluidic flow channel.

23. A system according to claim 22, wherein the sensor detector is configured to obtain a signal from a region of the sensor body that comprises predetermined authenticity or test data indicia.

24. A system according to claim 22, wherein the sensor body comprises sensors providing both positive and negative test controls.

25. A fluidic sensor device comprising:

a sensor body having an array of microfluidic flow channels, the sensor body having a plurality of layers residing one above another, including at least one working electrode layer, at least one reference electrode layer, and at least one counter electrode layer, wherein each layer has a corresponding array of apertures thereon sized and aligned so that the array of apertures of each of the layers define at least a portion of the fluidic flow channels.