Integrated membrane sensor

• US 8,753,894 B2
• Filed: 02/01/2008
• Issued: 06/17/2014
• Est. Priority Date: 02/01/2007
• Status: Active Grant

• Current Assignee: Diagnostic Biosensors LLC
• Original Assignee: Diagnostic Biosensors LLC
• Inventors: Tondra, Mark Christopher
• Primary Examiner(s): Hyun, Paul
• Assistant Examiner(s): EOM, ROBERT J
• Application Number:
  US12/024,987
• Publication Number:
  US 20080199971A1
• Time in Patent Office: 2,328 Days
• Field of Search: 422/50, 422/51, 422/68.1, 422/82.05, 422/82.08, 422/186, 436/526, 436/501, 436/518, 436/514, 435/287.2, 435/287.1
• US Class Current: 436/151
• CPC Class Codes: G01N 27/4145 specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01R 33/12 Measuring magnetic properties of articles or specimens of solids or fluids involving magnetic resonance G01R33/20
• CPC Class Codes: G01R 33/1269 of molecules labeled with magnetic beads magnetic particles for bio assay G01N33/54326

Abstract

An integrated microelectronic sensor is provided in a disposable flow membrane sensing device. The integrated sensors detect electromagnetic effect labels in flow detection zones above the sensor in the membrane. The labels are small particles that give off a detectable electromagnetic signal. They are commonly used for isolating and quantifying biochemical targets of interest. The sensors are fabricated using planar integrated circuit technologies. Sensors can detect labels of several types including magnetic, electric, and photonic. These types all have in common the fact that the sensor detects the label at a distance. Magnetoresistive sensors for detecting magnetic labels, and photodiodes for detecting photonic labels are described.

A system for using the sensors is described. There are disposable cartridges with a backing that supports the sensors and membrane is described. The integrated sensor in the cartridge is designed to be discarded after use. Also, label excitation sources are provided. The multi sensor array chip can be configured in order to detect labels in multiple zones, and to monitor progress of flow down a strip of membrane. These multiple label detection zones, using sandwich assay techniques, can quantify analyte concentration for many types of analytical samples. Also, the membrane can be micropatterned in order to provide multiple or unusually shaped flow paths.

Claims

1. An electromagnetic effect field detection system for measuring labels in a fluid comprising:

a planar substrate;

at least one electromagnetic effect field sensor formed on a first surface of said planar substrate;

a backing structure substantially supporting a second surface of said planar substrate where said second surface is on the opposite side of said planar substrate from said electromagnetic effect field sensor;

a flow permeable membrane supported substantially on said first surface of said planar substrate and covering said at least one electromagnetic effect field sensor;

wherein a thinnest dimension of said flow permeable membrane is perpendicular to said first surface of said planar substrate;

wherein a length and width of said flow permeable membrane are parallel to said first surface of said planar substrate;

one or more sensing lanes, each lane comprising;

a continuous flow path and intended flow direction through interconnected pores of said flow permeable membrane, said continuous flow path being substantially parallel to the length of said flow permeable membrane,

one or more label detection zones, where said label detection zones are disposed within said flow permeable membrane and positioned directly above said electromagnetic effect field sensor;

where the surface area of said flow permeable membrane is greater than that of said planar substrate;

wherein said continuous flow path further comprises features that divert flow by plugging some of said pores in said flow permeable membrane.

2. The system of claim 1 wherein said flow permeable membrane is bonded to the electromagnetic effect field sensor by a layer of adhesive material, and a portion of said flow permeable membrane extends beyond the edge of said planar substrate, in at least one direction.

3. The system of claim 2 further comprising a layer of material with low electrical conductivity interposed between the adhesive layer and the electromagnetic effect field sensor.

4. The system of claim 1 further comprising a layer of material with low electrical conductivity interposed between the electromagnetic effect field sensor and the flow permeable membrane.

5. The system of claim 1 wherein the electromagnetic effect field sensor detects an electromagnetic effect field emitted by a label in one of said label detection zones.

6. The system of claim 1 wherein some portions of said backing structure are substantially flush with said first surface of said planar substrate.