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Adhesive composition for use in an immunosensor

  • US 8,758,592 B2
  • Filed: 07/16/2012
  • Issued: 06/24/2014
  • Est. Priority Date: 09/30/2009
  • Status: Expired due to Fees
First Claim
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1. A method for measuring a blood sample, comprising:

  • providing;

    a reaction chamber and a detection chamber formed in a separator disposed between two electrodes;

    a fill chamber at least partially formed in the separator and one of the two electrodes, spaced a distance apart from the detection chamber, and overlapping at least a portion of the reaction chamber; and

    a vent at least partially formed in the separator and the two electrodes, spaced a distance apart from the reaction chamber, and overlapping at least a portion of the detection chamber;

    providing;

    an antibody-enzyme conjugate in a first buffer and magnetic beads linked to an antigen in a second buffer in the reaction chamber;

    ferricyanide, glucose, and a mediator in a dilute acid in the detection chamber;

    a first seal over a first side of the vent by way of a hydrophilic adhesive tape, wherein the hydrophilic adhesive tape also forms a wall of the fill chamber; and

    a second seal over a second side of the vent by way of a sealing component;

    providing a blood sample to the fill chamber, wherein at least a portion of the blood sample moves from the fill chamber to the reaction chamber;

    opening the vent after a pre-determined time by piercing at least one of the hydrophilic adhesive tape and the sealing component, thereby allowing portions of the blood sample containing the antibody-enzyme conjugate that is not bound to the magnetic beads to move to the detection chamber;

    catalyzing oxidation of the glucose in the detection chamber, which results in the formation of ferrocyanide;

    applying a potential between the two electrodes;

    electrochemically detecting a current from the ferrocyanide; and

    calculating a concentration of the antigen in the blood sample based on the signal detected.

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