Adhesive composition for use in an immunosensor
First Claim
1. A method for measuring a blood sample, comprising:
- providing;
a reaction chamber and a detection chamber formed in a separator disposed between two electrodes;
a fill chamber at least partially formed in the separator and one of the two electrodes, spaced a distance apart from the detection chamber, and overlapping at least a portion of the reaction chamber; and
a vent at least partially formed in the separator and the two electrodes, spaced a distance apart from the reaction chamber, and overlapping at least a portion of the detection chamber;
providing;
an antibody-enzyme conjugate in a first buffer and magnetic beads linked to an antigen in a second buffer in the reaction chamber;
ferricyanide, glucose, and a mediator in a dilute acid in the detection chamber;
a first seal over a first side of the vent by way of a hydrophilic adhesive tape, wherein the hydrophilic adhesive tape also forms a wall of the fill chamber; and
a second seal over a second side of the vent by way of a sealing component;
providing a blood sample to the fill chamber, wherein at least a portion of the blood sample moves from the fill chamber to the reaction chamber;
opening the vent after a pre-determined time by piercing at least one of the hydrophilic adhesive tape and the sealing component, thereby allowing portions of the blood sample containing the antibody-enzyme conjugate that is not bound to the magnetic beads to move to the detection chamber;
catalyzing oxidation of the glucose in the detection chamber, which results in the formation of ferrocyanide;
applying a potential between the two electrodes;
electrochemically detecting a current from the ferrocyanide; and
calculating a concentration of the antigen in the blood sample based on the signal detected.
8 Assignments
0 Petitions
Accused Products
Abstract
An adhesive composition for use in devices and methods for measuring a presence or a concentration of a particular component, such as an antigen, in a sample, such as blood, are provided. In one exemplary embodiment of an adhesive composition, the composition includes an adhesive, water, a poloxamer, and an anticoagulant. The adhesive can include particular properties, such as being hydrophilic, pressure-sensitive, heat-activated, and/or water soluble. The adhesive is particularly useful because it can help improve the flow of sample a device. For example, when the device is an immunosensor, the adhesive can help prevent the blood from clotting in chambers of the immunosensor. This results in a more efficient and accurate determination of the concentration of the sample. Methods of making the composition and device in which the composition can be used are provided, as are methods of using the same.
-
Citations
6 Claims
-
1. A method for measuring a blood sample, comprising:
-
providing; a reaction chamber and a detection chamber formed in a separator disposed between two electrodes; a fill chamber at least partially formed in the separator and one of the two electrodes, spaced a distance apart from the detection chamber, and overlapping at least a portion of the reaction chamber; and a vent at least partially formed in the separator and the two electrodes, spaced a distance apart from the reaction chamber, and overlapping at least a portion of the detection chamber; providing; an antibody-enzyme conjugate in a first buffer and magnetic beads linked to an antigen in a second buffer in the reaction chamber; ferricyanide, glucose, and a mediator in a dilute acid in the detection chamber; a first seal over a first side of the vent by way of a hydrophilic adhesive tape, wherein the hydrophilic adhesive tape also forms a wall of the fill chamber; and a second seal over a second side of the vent by way of a sealing component; providing a blood sample to the fill chamber, wherein at least a portion of the blood sample moves from the fill chamber to the reaction chamber; opening the vent after a pre-determined time by piercing at least one of the hydrophilic adhesive tape and the sealing component, thereby allowing portions of the blood sample containing the antibody-enzyme conjugate that is not bound to the magnetic beads to move to the detection chamber; catalyzing oxidation of the glucose in the detection chamber, which results in the formation of ferrocyanide; applying a potential between the two electrodes; electrochemically detecting a current from the ferrocyanide; and calculating a concentration of the antigen in the blood sample based on the signal detected. - View Dependent Claims (2, 3, 4, 5, 6)
-
Specification