Microfluidic diagnostic device

• US 8,758,704 B2
• Filed: 07/29/2010
• Issued: 06/24/2014
• Est. Priority Date: 08/11/2009
• Status: Active Grant

• Current Assignee: Baril Corporation
• Original Assignee: Baril Corporation
• Inventors: Baril, Daniel J.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Kingan, Timothy G
• Application Number:
  US12/846,692
• Publication Number:
  US 20110038767A1
• Time in Patent Office: 1,426 Days
• Field of Search: 422500-507, 156/275.5, 156/277, 600/345, 600/347, 600/300, 600/309, 600/310, 600/316
• US Class Current: 422/503
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B29C 65/482 Drying adhesives, e.g. solvent based adhesives
• CPC Class Codes: B29C 65/4825 Pressure sensitive adhesives
• CPC Class Codes: B29C 65/483 Reactive adhesives, e.g. chemically curing adhesives
• CPC Class Codes: B29C 65/4835 Heat curing adhesives
• CPC Class Codes: B29C 65/4845 Radiation curing adhesives, e.g. UV light curing adhesives
• CPC Class Codes: B29C 65/522 by spraying, e.g. by flame spraying
• CPC Class Codes: B29C 65/526 by printing or by transfer from the surfaces of elements carrying the adhesive, e.g. using brushes, pads, rollers, stencils or silk screens
• CPC Class Codes: B29C 66/1122 Single lap to lap joints, i.e. overlap joints B29C66/45, B29C66/472, B29C66/52272 take precedence
• CPC Class Codes: B29C 66/45 Joining of substantially the whole surface of the articles methods or apparatus for laminating B32B37/00
• CPC Class Codes: B29C 66/71 characterised by the composition of the plastics material of the parts to be joined welding bar compositions B29C65/125
• CPC Class Codes: B29C 66/73171 Hydrophilicity
• CPC Class Codes: B29C 66/73175 Hydrophobicity
• CPC Class Codes: B29K 2067/00 Use of polyesters or derivatives thereof, as moulding material
• CPC Class Codes: B29L 2031/737 Articles provided with holes, e.g. grids, sieves nets B29L2028/00
• CPC Class Codes: B29L 2031/756 : Microarticles, nanoarticles
• CPC Class Codes: B81B 2201/058 : Microfluidics not provided for in B81B2201/051 - B81B2201/054
• CPC Class Codes: B81C 2201/019 : Bonding or gluing multiple substrate layers
• CPC Class Codes: B81C 99/0095 : Aspects relating to the manufacture of substrate-free structures, not covered by groups B81C99/008 - B81C99/009
• CPC Class Codes: Y10T 156/10 : Methods of surface bonding and/or assembly therefor
• CPC Class Codes: Y10T 428/24843 : with heat sealable or heat releasable adhesive layer
• CPC Class Codes: Y10T 428/24851 : Intermediate layer is discontinuous or differential

Abstract

A method of making a microfluidic diagnostic device for use in the assaying of biological fluids, whereby a layer of adhesive in a channel pattern is printed onto a surface of a base sheet and a cover sheet is adhered to the base sheet with the adhesive. The layer of adhesive defines at least one channel, wherein the channel passes through the thickness of the adhesive layer.

Claims

1. A method of making a microfluidic diagnostic device, the method comprising:

printing a channel pattern onto a surface of a base sheet with one or more non-adhesive layers prior to printing a layer of adhesive onto the channel pattern, wherein the pattern defines at least one channel passing through the thickness of the adhesive; and

adhering a cover sheet to the base sheet with the adhesive to enclose the at least one channel when the cover sheet is adhered to the base sheet and wherein at least a portion of the channel has a mean width less than 1.0 mm, and wherein the cover sheet comprises at least one fluid sample inlet portal and at least one vent aperture each of which is configured to be in fluid communication with the at least one channel.

2. The method set forth in claim 1, wherein the adhesive defines a plurality of channels and wherein adjacent channels are separated by adhesive and by non-channel space that is void of adhesive.

3. The method set forth in claim 1, wherein either the base sheet or the cover sheet or both comprises a hydrophilic surface in contact with the at least one channel.

4. The method set forth in claim 1, wherein at least a portion of the channel has a mean width less than 0.5 mm.

5. The method set forth in claim 1, wherein the adhesive layer has a thickness ranging from 0.0025 mm to 0.25 mm.

6. The method set forth in claim 1, wherein the channel forms a fluid flow path within which a fluid sample to be assayed can pass via capillary action.

7. The method set forth in claim 1, wherein the adhesive is non-hydrophilic.

8. The method set forth in claim 1, wherein the adhesive is pressure sensitive.

9. The method set forth in claim 1, wherein the adhesive is printed to the base sheet by a technique selected from the group consisting of rotary or flat screen printing, pad printing, spray printing, offset printing, flexo graphic printing and transfer printing.

10. A microfluidic diagnostic device adapted for use in the assaying of biological fluids, the device comprising:

a base sheet;

one or more non-adhesive layers disposed on a surface of the base sheet in a channel pattern, and an adhesive layer disposed on the channel pattern wherein the adhesive defines at least one fluid transport channel, the channel having a mean width less than 0.6 mm;

a cover sheet adhered to the base sheet by means of the adhesive, wherein the cover sheet encloses the at least one channel when the cover sheet is adhered to the base sheet; and

wherein either the base sheet or the cover sheet or both comprise a hydrophilic surface in contact with the adhesive, and wherein the cover sheet comprises at least one fluid sample inlet portal and at least one vent aperture each of which is configured to be in fluid communication with the at least one channel.