Methods for genetic control of insect infestation in plants and compositions thereof
First Claim
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1. A nucleic acid molecule comprising a recombinant polynucleotide selected from the group consisting of:
- (a) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO;
841;
(b) a polynucleotide comprising at least 95% sequence identity to SEQ ID NO;
841;
(c) a fragment of at least 23 contiguous nucleotides of a nucleic acid sequence of SEQ ID NO;
841, wherein ingestion by a corn rootworm of a double stranded ribonucleotide sequence comprising at least one strand that is complementary to said fragment inhibits the growth of said corn rootworm; and
(d) a complement of the sequence of (a), (b), or (c);
wherein said nucleic acid molecule further comprises a heterologous promoter operably linked to said recombinant polynucleotide.
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Abstract
The present invention relates to control of pest infestation by inhibiting one or more biological functions. The invention provides methods and compositions for such control, By feeding one or more recombinant double stranded RNA molecules provided by the invention to the pest, a reduction in pest infestation is obtained through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.
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Citations
32 Claims
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1. A nucleic acid molecule comprising a recombinant polynucleotide selected from the group consisting of:
- (a) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO;
841;
(b) a polynucleotide comprising at least 95% sequence identity to SEQ ID NO;
841;
(c) a fragment of at least 23 contiguous nucleotides of a nucleic acid sequence of SEQ ID NO;
841, wherein ingestion by a corn rootworm of a double stranded ribonucleotide sequence comprising at least one strand that is complementary to said fragment inhibits the growth of said corn rootworm; and
(d) a complement of the sequence of (a), (b), or (c);
wherein said nucleic acid molecule further comprises a heterologous promoter operably linked to said recombinant polynucleotide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
- (a) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO;
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16. A method for controlling corn rootworm infestation comprising providing in the diet of a corn rootworm an agent comprising a first polynucleotide sequence that functions upon ingestion by the corn rootworm to inhibit a biological function within said corn rootworm, wherein said polynucleotide sequence exhibits from about 95 to about 100% nucleotide sequence identity along at least from about 19 to about 25 contiguous nucleotides to a coding sequence derived from said corn rootworm and is hybridized to a second polynucleotide sequence that is complementary to said first polynucleotide sequence, and wherein said coding sequence derived from said corn rootworm is selected from the group consisting of SEQ ID NO:
- 841, and the complement thereof.
- View Dependent Claims (17)
Specification