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Transformation of algal cells

  • US 8,759,615 B2
  • Filed: 02/13/2012
  • Issued: 06/24/2014
  • Est. Priority Date: 06/06/2008
  • Status: Expired due to Fees
First Claim
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1. A method for genetically modifying an algal cell, the method comprising:

  • adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, the nucleic acid including a gene involved in lipid biosynthesis;

    introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell; and

    selecting a colony that includes the transformed algal cell;

    wherein the electrical pulse is applied with a field strength of higher than approximately 7500 V/cm; and

    wherein the algal cell is of genus Nannochloropsis.

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