Enhancing fermentation of starch- and sugar-based feedstocks

• US 8,765,430 B2
• Filed: 12/31/2012
• Issued: 07/01/2014
• Est. Priority Date: 02/10/2012
• Status: Expired due to Fees

• Current Assignee: Apalta Tehnoloogia Ou
• Original Assignee: SweetWater Energy, Inc
• Inventors: Parekh, Sarad, Felice, Carl P., Stauber, Benjamin
• Primary Examiner(s): Weber, Jon P
• Assistant Examiner(s): CONSTANTINE, CHARLES Z
• Application Number:
  US13/731,633
• Publication Number:
  US 20130210101A1
• Time in Patent Office: 547 Days
• US Class Current: 435/165
• CPC Class Codes: C12P 2203/00 Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source ethanol C12P7/10
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

Provided are methods, systems, and compositions for increasing the rate and/or yield of fermentation processes using blended feedstocks. Also provided are methods, systems, and compositions for decreasing the yield of one or more undesirable products during fermentation.

Claims

1. A method of producing one or more fermentation end-products comprising:

(a) combining a composition comprising a biomass and a liquid, with a solution comprising a cellulosic hydrolysate comprising about 10% to about 70% w/v of C6 monosaccharides to produce a blended feedstock;

(b) contacting the blended feedstock with one or more biocatalysts; and

(c) fermenting the blended feedstock to produce one or more fermentation end-products;

wherein the biomass comprises corn, corn mash, sugar cane, sugar beets, sugar palms, sweet sorghum, nypa palm, cassava, rice, milo, sorghum, sweet potatoes, wheat, molasses, tubers, roots, stems, whole grains, barley, rye, milo, sago, cassava, tapioca, rice, peas, beans, potatoes, beets, fruits, or a combination thereof, and

wherein the cellulosic hydrolysate is prepared by a method comprising

pretreating liqnocellulosic material with hot water or dilute acid to solubilize hemicellulose in the liqnocellulosic material,

substantially separating the solubilized hemicellulose from remaining lignocellulosic solids, and

enzymatically hydrolyzing cellulose in the remaining lignocellulosic solids to obtain the cellulosic hydrolysate,

wherein less than 20% of the total saccharides in the cellulsoic hydrolysate are C5 saccharides.

2. The method of claim 1, wherein a yield of at least one of the one or more fermentation end-products is increased relative to fermentation of the biomass without the cellulosic hydrolysate.

3. The method of claim 1, wherein a yield of at least one of the one or more fermentation end-products is increased by at least 5% relative to fermentation of the biomass without the cellulosic hydrolysate.

4. The method of claim 1, wherein at least one of the one or more fermentation end-products is produced at a rate that is faster relative to fermentation of the first biomass without the cellulosic hydrolysate.

5. The method of claim 1, wherein the one or more fermentation end-products comprise one or more alcohols.

6. The method of claim 1, wherein the one or more fermentation end-products comprise ethanol.

7. The method of claim 1, wherein the method is a fed-batch fermentation wherein the solution is added over time.

8. The method of claim 7, wherein a yield of one or more by-products is decreased relative to a non-fed batch fermentation.

9. The method of claim 8, wherein the one or more by-products comprise methanol, glycol, glycerol, erythritol, threitol, arabitol, xylitol, ribitol, mannitol, sorbitol, dulcitol, fucitol, iditol, inositol, volemitol, isomalt, maltitol, lactitol, polyglycitol, or a combination thereof.

10. The method of claim 8, wherein the concentration of C6 monosaccharides in the solution is from about 20% to about 55% w/v.

11. The method of claim 1, wherein the C6 monosaccharides are at a concentration in the solution that differs from a monosaccharides equivalent concentration of saccharides in the composition by less than 50%.

12. The method of claim 11, wherein the composition and the solution are combined in about an 80:

20 v/v ratio.

13. The method of claim 11, wherein the composition and the solution are combined in about a 90:

10 v/v ratio.

14. The method of claim 1, wherein the C6 monosaccharides are at a concentration in the solution that differs from a monosaccharides equivalent concentration of saccharides in the composition by less than 40%.

15. The method of claim 1, wherein the C6 monosaccharides are at a concentration in the solution that differs from a monosaccharides equivalent concentration of saccharides in the composition by less than 30%.

16. The method of claim 1, wherein the C6 monosaccharides are at a concentration in the solution that differs from a monosaccharides equivalent concentration of saccharides in the composition by less than 20%.

17. The method of claim 1, wherein less than 10% of the total saccharides in the cellulosic hydrolysate are C5 saccharides.

18. The method of claim 1, wherein about 0.1% to about 10% of the total saccharides in the cellulosic hydrolysate are C5 saccharides.

19. The method of claim 1, wherein combining the solution and the composition occurs during fermentation at a rate of about 0.01-5 mL/min of the solution per L of the composition.

20. The method of claim 1, wherein the one or more biocatalysts comprise one or more fermenting microorganisms.

21. The method of claim 20, wherein the one or more fermenting microorganisms comprise a C6 fermenting microorganism.

22. The method of claim 20, wherein the one or more fermenting microorganisms comprise one or more yeast strains.

23. The method of claim 22, wherein the one or more yeast strains comprise a Saccharomyces or a Zygosaccharomyces strain.

24. The method of claim 20, wherein the one or more biocatalysts further comprise one or more enzymes.

25. The method of claim 24, wherein the one or more enzymes comprise an endoglucanase, an exoglucanase, a cellobiohydrolase, a cellulase, a beta-glucosidase, a glycoside hydrolase, a glycosyltransferase, a lyase, an esterase, a glucamylase, or a combination thereof.