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Alcohol dehydrogenases (ADH) useful for fermentive production of lower alkyl alcohols

  • US 8,765,433 B2
  • Filed: 12/29/2010
  • Issued: 07/01/2014
  • Est. Priority Date: 12/29/2009
  • Status: Active Grant
First Claim
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1. A recombinant microbial host cell comprising:

  • a biosynthetic pathway for production of isobutanol, the biosynthetic pathway comprising a substrate to product conversion catalyzed by a polypeptide with alcohol dehydrogenase activity, wherein the polypeptide with alcohol dehydrogenase activity has at least 90% identity to the amino acid sequence of SEQ ID NO;

    31 and either or both of the following characteristics;

    (a) the KM value for isobutyraldehyde is lower for said polypeptide relative to a control polypeptide having the amino acid sequence of SEQ ID NO;

    26; and

    (b) the kcat/KM value isobutyraldehyde for said polypeptide is higher relative to a control polypeptide having the amino acid sequence of SEQ ID NO;

    26.

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