Methods of obtaining freshwater or saltwater algae products enriched in glutelin proteins
First Claim
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1. A method of selectively extracting glutelin proteins from a wet freshwater or saltwater algal biomass or algal culture comprised of intact algal cells, the method comprising:
- a. providing a wet freshwater or saltwater algal biomass or algal culture comprised of intact algal cells;
b. heating the freshwater or saltwater algal biomass or algal culture to generate a heated slurry comprising a liquid phase and a solid phase, wherein the heated slurry is heated to a temperature below the boiling point of the extraction mixture;
c. raising the pH of the heated slurry to increase the concentration of glutelin proteins in the liquid phase; and
d. separating at least a portion of said liquid phase comprising glutelin proteins from the solid phase, wherein the solid phase comprises lipids.
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Abstract
Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
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14 Claims
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1. A method of selectively extracting glutelin proteins from a wet freshwater or saltwater algal biomass or algal culture comprised of intact algal cells, the method comprising:
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a. providing a wet freshwater or saltwater algal biomass or algal culture comprised of intact algal cells; b. heating the freshwater or saltwater algal biomass or algal culture to generate a heated slurry comprising a liquid phase and a solid phase, wherein the heated slurry is heated to a temperature below the boiling point of the extraction mixture; c. raising the pH of the heated slurry to increase the concentration of glutelin proteins in the liquid phase; and d. separating at least a portion of said liquid phase comprising glutelin proteins from the solid phase, wherein the solid phase comprises lipids. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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