Systems and methods for extracting lipids from and dehydrating wet algal biomass

• US 8,765,983 B2
• Filed: 01/03/2011
• Issued: 07/01/2014
• Est. Priority Date: 10/30/2009
• Status: Expired due to Fees

• Current Assignee: Aurora Algae, Incorporated (Reliance Industries Limited)
• Original Assignee: Aurora Algae, Incorporated (Reliance Industries Limited)
• Inventors: Fleischer, Daniel, Jukic, Marko, Thompson, Andrew, Radaelli, Guido
• Primary Examiner(s): Carr, Deborah D
• Application Number:
  US12/983,767
• Publication Number:
  US 20110196163A1
• Time in Patent Office: 1,275 Days
• Field of Search: 554/8, 554/20, 554/21, 554/206
• US Class Current: 554/21
• CPC Class Codes: C11B 1/10 by extracting
• CPC Class Codes: C11B 1/106 using ultra-sounds

Abstract

Exemplary methods include centrifuging a wet algal biomass to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a centrifuged algal biomass, mixing the centrifuged algal biomass with an amphiphilic solvent to result in a mixture, heating the mixture to result in a dehydrated, defatted algal biomass, separating the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids, evaporating the amphiphilic solvent from the water and the lipids, and separating the water from the lipids. The amphiphilic solvent may be selected from a group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde. Other exemplary methods include filtering a wet algal biomass through a membrane to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a filtered algal biomass.

Claims

1. A method comprising:

mixing algal biomass with an amphiphilic solvent;

separating the amphiphilic solvent from algal solids, or from any part of the algal biomass not dissolved in the amphiphilic solvent, to result in amphiphilic solvent, water and lipids;

evaporating most or substantially all of the amphiphilic solvent from the water and the lipids, to result in a mixture of the water and the lipids; and

separating the lipids from the mixture.

2. The method of claim 1, wherein the amphiphilic solvent is selected from a group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde.

3. A method comprising:

filtering a wet algal biomass through a membrane to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a filtered algal biomass;

mixing the filtered algal biomass with an amphiphilic solvent to result in a mixture;

heating the mixture to result in a dehydrated, defatted algal biomass;

separating the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids;

evaporating the amphiphilic solvent from the water and the lipids; and

separating the water from the lipids.

4. The method of claim 3, wherein the wet algal biomass is filtered to increase the solid content to approximately 30%.

5. The method of claim 3, wherein the amphiphilic solvent is selected from a group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde.

6. The method of claim 3, wherein the mixture is heated in a pressurized reactor.

7. The method of claim 6, wherein the pressurized reactor is a batch or a continuous pressurized reactor.

8. The method of claim 3, wherein the mixture is heated with microwaves, ultrasound, steam, or hot oil.

9. The method of claim 3, wherein the amphiphilic solvent is separated from the dehydrated, defatted algal biomass via membrane filtration to result in amphiphilic solvent, water and lipids.

10. The method of claim 3, wherein the amphiphilic solvent is separated from the dehydrated, defatted algal biomass via centrifugation to result in amphiphilic solvent, water and lipids.

11. The method of claim 3, wherein the separating includes decanting the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids.

12. The method of claim 3, wherein the separating of the water from the lipids includes adding a nonpolar solvent.

13. The method of claim 12, wherein the nonpolar solvent is propane, butane, pentane, hexane, butene, propene, naphtha or gasoline.

14. The method of claim 3, wherein the separating of the water from the lipids includes decanting the lipids without a nonpolar solvent.

15. The method of claim 3, wherein the separating of the water from the lipids includes adding a nonpolar solvent in a continuous liquid-liquid extractor.

16. The method of claim 15, wherein the nonpolar solvent is evaporated from the lipids by distillation or flash evaporation.

17. The method of claim 3, wherein the separating of the water from the lipids includes adding a nonpolar solvent in a batch vessel and decanting the batch vessel.

18. The method of claim 17, wherein the nonpolar solvent is evaporated from the lipids by distillation or flash evaporation.

19. The method of claim 3, wherein the wet algal biomass is centrifuged to increase the solid content to approximately 30%.

20. The method of claim 3, wherein the evaporating the amphiphilic solvent from the water and the lipids is performed by flash evaporation, distillation or by pervaporation.