Module for processing a biological sample, biochip kit, and use of the module

• US 8,771,609 B2
• Filed: 01/16/2007
• Issued: 07/08/2014
• Est. Priority Date: 01/17/2006
• Status: Active Grant

• Current Assignee: Boehringer Ingelheim Vetmedica GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Original Assignee: Siemens AG
• Inventors: Ehben, Thomas, Gumbrecht, Walter, Stanzel, Manfred, Zilch, Christian
• Primary Examiner(s): Nagpaul, Jyoti
• Application Number:
  US11/653,230
• Publication Number:
  US 20070166192A1
• Time in Patent Office: 2,730 Days
• Field of Search: 422 99-100, 422500-504, 422/50, 241 1- 2, 436/180
• US Class Current: 422/501
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/565 Seals in general F16L
• CPC Class Codes: G01N 1/40 Concentrating samples
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A module for processing a biological sample for an analysis test is disclosed. The processing module includes, in at least one embodiment, an interface at which the processing module can be connected to a cartridge with a lab-on-a-chip, in which cartridge the analysis steps are carried out. A biochip kit is also disclosed. In at least one embodiment, the biochip kit includes one or more processing modules which are intended for different sample materials and which can be connected to the same cartridge type.

Claims

1. A module for processing a biological sample for a test in which a target substance in the sample is at least one of detected and quantified, the processing module comprising:

a hollow space, wherein the hollow space includes a narrowing having an first opening at a tip of the narrowing;

a displaceable plunger arranged in the hollow space, the displaceable plunger being configured to draw up the biological sample;

at least one of a first membrane and a first partition wall that seals of the first opening in a leaktight manner when a cartridge with a miniaturized laboratory is not connected to the processing module, and that allows a fluidic connection between the cartridge and the processing module when the cartridge is connected to the processing module; and

a first interface at which the processing module is connectable to the cartridge with the miniaturized laboratory, the cartridge including,

a plurality of channels configured to perform analysis steps for the at least one of detection and quantification of the target substance, and

a second interface including a second opening sealed by at least one of a second membrane and a second partition wall,

wherein the cartridge with the miniaturized laboratory is one selected from a biochip miniaturized to a check card format and a lab-on-chip,

an inside of the at least one of the first membrane and the first partition wall includes a spike adapted to pierce the at least one of the second membrane and the second partition wall upon connection of the processing module to the cartridge, and

the first interface is connected to the hollow space.

2. The processing module as claimed in claim 1, wherein the cartridge is configured to enable

at least one of concentration, extraction and multiplication of cells,

at least one of concentration, extraction and amplification of molecules,

at least one of homogenization and liquefaction of the sample,

cell lysis, and

combinations thereof.

3. The processing module as claimed in claim 1, wherein the hollow space contains spherules which, on their surface, are provided with binding partners of the target substance.

4. The processing module as claimed in claim 3, wherein the spherules are magnetic beads which, by way of a magnetic field, are transferable from the processing module through the first interface into the cartridge.

5. The processing module as claimed in claim 3, wherein the first interface is configured to transfer the spherules, when the processing module is connected to the cartridge, in order to transfer the processed sample from the processing module to the cartridge.

6. The processing module as claimed in claim 1, wherein the first interface is adapted to permit at least one of a plug-type, screw-type, tube-type and bayonet-type connection to the cartridge.

7. The processing module as claimed in claim 1, wherein the spike is adapted to establish a connection between the cartridge and the processing module.

8. The processing module as claimed in claim 1, wherein reagents necessary for processing are stored in the processing module in a liquid or dry state.

9. The processing module as claimed in claim 1, wherein, when connecting the processing module to the cartridge, a fluidic connection is established for transferring the processed sample from the processing module to the cartridge.

10. A method, comprising:

using the processing module as claimed in claim 1 for processing the biological sample for the test in the cartridge with the miniaturized laboratory, wherein the analysis steps necessary for the at least one of detection and quantifying of a target substance are carried out in the cartridge.

11. The processing module as claimed in claim 1, wherein the hollow space comprises a funnel-shaped end.

12. The processing module as claimed in claim 1, further comprising a seal at the first opening of the narrowing, wherein the tip of the narrowing is at least one of embedded within or contacting the seal.

13. The processing module as claimed in claim 1, further comprising an agitator in the hollow space.

14. The processing module as claimed in claim 13, wherein the agitator is drivable by externally applied magnetic fields.

15. The processing module as claimed in claim 14, wherein the agitator has cutting blades for homogenization of the sample.

16. The processing module as claimed in claim 14, wherein the agitator is additionally provided with cutting blades for homogenization of the sample.

17. The processing module as claimed in claim 1, wherein the narrowing of the hollow space includes a tubular portion extending past and enclosing the tip and the first opening in the tip is closed by the at least one of the first membrane and the first partition extending between sidewalls of the tubular portion.

18. The processing module as claimed in claim 17, wherein the first interface of the cartridge includes a sidewall extending from a surface of the cartridge and the sidewall is configured to interface with the tubular portion to provide a physical connection between the cartridge and the hollow space.

19. The processing module as claimed in claim 1, wherein the processing module is configured to process a larger volume of the biological sample than the cartridge.

20. The processing module of claim 1, wherein the at least one of the first membrane and the first partition wall is configured to surround the tip of the narrowing, when the processing module is connected to the cartridge.