Methods of using adipose tissue-derived cells in augmenting autologous fat transfer
First Claim
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1. A method of augmenting autologous fat transfer to a subject, comprising:
- removing a first portion of adipose tissue that comprises a cell population comprising adipose-derived stem cells and progenitor from said subject;
introducing the removed adipose tissue into a self-contained cell processing unit configured to maintain a closed pathway;
isolating said cell population that comprises adipose-derived stem cells and progenitor cells from mature adipocytes and connective tissue present in the removed adipose tissue so as to obtain a substantially isolated cell population comprising disaggregated adipose-derived stem cells and progenitor cells, while maintaining a closed pathway;
concentrating said substantially isolated cell population comprising disaggregated adipose-derived stem cells so as to obtain a concentrated cell population that comprises disaggregated adipose-derived stem cells and progenitor cells, while maintaining a closed pathway;
mixing said concentrated cell population comprising adipose-derived stem cells and progenitor cells with a second portion of unprocessed adipose tissue comprising intact, non-disaggregated tissue fragments obtained from said subject, so as to obtain an adipose tissue composition comprising a mixture of said concentrated cell population comprising disaggregated adipose-derived stem cells and progenitor cells and said unprocessed adipose tissue comprising intact, non-disaggregated tissue fragments tissue; and
reintroducing said adipose tissue composition into said subject.
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Abstract
Methods of treating patients for conditions such as breast augmentation, soft tissue defects, and urinary incontinence, are described. The methods include removing adipose tissue from a patient, processing a portion of the adipose tissue to obtain a substantially isolated population of cells comprising disaggregated adipose-derived stem cells, mixing the concentrated population of adipose-derived cells comprising disaggregated adipose-derived cells with a portion of unprocessed, intact, non-disaggregated adipose tissue to form a composition, and administering the composition to the patient from which the adipose tissue was removed.
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Citations
14 Claims
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1. A method of augmenting autologous fat transfer to a subject, comprising:
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removing a first portion of adipose tissue that comprises a cell population comprising adipose-derived stem cells and progenitor from said subject; introducing the removed adipose tissue into a self-contained cell processing unit configured to maintain a closed pathway; isolating said cell population that comprises adipose-derived stem cells and progenitor cells from mature adipocytes and connective tissue present in the removed adipose tissue so as to obtain a substantially isolated cell population comprising disaggregated adipose-derived stem cells and progenitor cells, while maintaining a closed pathway; concentrating said substantially isolated cell population comprising disaggregated adipose-derived stem cells so as to obtain a concentrated cell population that comprises disaggregated adipose-derived stem cells and progenitor cells, while maintaining a closed pathway; mixing said concentrated cell population comprising adipose-derived stem cells and progenitor cells with a second portion of unprocessed adipose tissue comprising intact, non-disaggregated tissue fragments obtained from said subject, so as to obtain an adipose tissue composition comprising a mixture of said concentrated cell population comprising disaggregated adipose-derived stem cells and progenitor cells and said unprocessed adipose tissue comprising intact, non-disaggregated tissue fragments tissue; and reintroducing said adipose tissue composition into said subject. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification