Marine bacterium of metabolizing 3,6-anhydro-L-galactose and use of the same

• US 8,771,996 B2
• Filed: 07/25/2012
• Issued: 07/08/2014
• Est. Priority Date: 07/26/2011
• Status: Active Grant

• Current Assignee: Korea University Research and Business Foundation (Korea University)
• Original Assignee: Korea University Research and Business Foundation (Korea University)
• Inventors: Kim, Kyoung Heon, Choi, In Geol, Yun, Eun Ju, Lee, Sae Young
• Primary Examiner(s): NOAKES, SUZANNE MARIE
• Application Number:
  US13/557,803
• Publication Number:
  US 20130102036A1
• Time in Patent Office: 713 Days
• Field of Search: None
• US Class Current: 435/105
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor

Abstract

Disclosed is a novel marine microorganism which can metabolize 3,6-anhydro-L-galactose and use of the same. The novel marine microorganism belongs to Vibrio sp. which utilizes 3,6-anhydro-L-galactose (3,6-L-AHG) as a single carbon source to convert 3,6-L-AHG to ribose and 3,6-anhydrogalactonic acid.

Claims

1. A marine microorganism, Vibrio sp. EJY3, that has been grown using 3,6-anhydro-L-galactose (3,6-L-AHG) as a single carbon source and is deposited under the Accession No. KCTC 11976BP.

2. The marine microorganism, Vibrio sp. EJY3, according to claim 1, wherein the marine microorganism metabolizes 3,6-L-AHG to produce ribose and 3,6-anhydro galactonic acid.

3. A method of culturing a novel marine microorganism, Vibrio sp. EIY3, according to claim 1, the method comprising:

culturing the marine microorganism, Vibrio sp. EIY3, using 3,6-L-AHG as a carbon source.

4. A method of producing ribose and 3,6-anhydrogalactonic acid, comprising:

producing ribose and 3,6-anhydrogalactonic acid by reacting a culture broth of the novel marine microorganism, Vibrio sp. EIY3, according to claim 1 or a crude enzyme extract thereof with 3,6-L-AHG.

5. The method according to claim 4, wherein the crude enzyme extract includes a water soluble protein obtained by sonicating and centrifuging the culture broth of the novel marine microorganism.

6. The method acid according to claim 4, wherein the reaction is conducted by further adding NADH.