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β-Glucosidase variants

  • US 8,772,010 B2
  • Filed: 06/16/2010
  • Issued: 07/08/2014
  • Est. Priority Date: 06/16/2009
  • Status: Active Grant
First Claim
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1. A method of producing a variant Myceliophthora thermophila β

  • -glucosidase polypeptide with improved thermoactivity, comprising;

    a) identifying a first performance sensitive position (PSP) in a target Myceliophthora thermophila β

    -glucosidase polypeptide, comprising the steps of;

    i) aligning the primary sequence of the target Myceliophthora thermophila β

    -glucosidase polypeptide with one or both of SEQ ID NO;

    53 or portion thereof, and SEQ ID NO;

    54, or portion thereof, andii) identifying a position in the target Myceliophthora thermophila β

    -glucosidase polypeptide that corresponds in the alignment to a PSP in the one or more β

    -glucosidase polypeptides, wherein the position so identified is a PSP; and

    b) expressing a variant Myceliophthora thermophila β

    -glucosidase polypeptide in which the residue at the first performance sensitive position is replaced with a thermoactivity enhancing residue,wherein the variant Myceliophthora thermophila β

    -glucosidase polypeptide has greater thermoactivity than the target Myceliophthora thermophila β

    -glucosidase polypeptide.

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