Methods and compositions for inactivating alpha 1,6 fucosyltransferase (FUT8) gene expression
First Claim
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1. A zinc finger DNA-binding domain, wherein the DNA-binding domain comprises four zinc finger recognition regions ordered F1 to F4 from N-terminus to C-terminus, and wherein F1, F2, F3, and F4 comprise the following amino acid sequences:
- F1;
QSSDLSR (SEQ ID NO;
9);
F2;
TSGNLTR (SEQ ID NO;
10);
F3;
RSDDLSK (SEQ ID NO;
11); and
F4;
DRSALAR (SEQ ID NO;
12).
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Abstract
Disclosed herein are methods and compositions for inactivating a FUT8 gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.
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Citations
22 Claims
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1. A zinc finger DNA-binding domain, wherein the DNA-binding domain comprises four zinc finger recognition regions ordered F1 to F4 from N-terminus to C-terminus, and wherein F1, F2, F3, and F4 comprise the following amino acid sequences:
-
F1;
QSSDLSR (SEQ ID NO;
9);F2;
TSGNLTR (SEQ ID NO;
10);F3;
RSDDLSK (SEQ ID NO;
11); andF4;
DRSALAR (SEQ ID NO;
12). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A zinc finger DNA-binding domain, wherein the DNA-binding domain comprises five zinc finger recognition regions ordered F1 to F5 from N-terminus to C-terminus, and wherein F1, F2, F3, F4, and F5 comprise the following amino acid sequences:
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FI;
RSDNLRE (SEQ ID NO;
26);F2;
NNTQLIE (SEQ ID NO;
27);F3;
TSSILSR (SEQ ID NO;
28);F4 RSDNLSA (SEQ ID NO;
29); andF5;
RKDTRIT (SEQ ID NO;
30). - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification