Automated method for sample distribution and isolation of an analyte with a solid support

• US 8,772,036 B2
• Filed: 06/27/2011
• Issued: 07/08/2014
• Est. Priority Date: 06/29/2010
• Status: Active Grant

• Current Assignee: Roche Molecular Systems Inc (Roche Holding AG)
• Original Assignee: Roche Molecular Systems Inc (Roche Holding AG)
• Inventors: Frank, Paul, Gisler, Andreas, Huesler, Robert, Knobel, Rolf, Mueller, Siegfried, Schnieper, Urs, Trueeb, Heinz
• Primary Examiner(s): Soderquist, Arlen
• Application Number:
  US13/169,559
• Publication Number:
  US 20120164735A1
• Time in Patent Office: 1,107 Days
• Field of Search: 422/63, 422/67, 422509-511, 422514-516, 436/43, 436/49, 436/54, 436/180
• US Class Current: 436/43
• CPC Class Codes: B01L 3/0275 Interchangeable or disposable dispensing tips
• CPC Class Codes: G01N 2035/00326 Analysers with modular structure
• CPC Class Codes: G01N 2035/103 using disposable tips
• CPC Class Codes: G01N 2035/1069 by adjusting the spacing between multiple probes of a single transferring head
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: G01N 35/1067 for transfer to or from containers having different spacing
• CPC Class Codes: G01N 35/1074 arranged in a two-dimensional array
• CPC Class Codes: G01N 35/1095 for supplying the samples to flow-through analysers for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/114998 with treatment or replacement of aspirator element [e.g., cleaning, etc.]
• CPC Class Codes: Y10T 436/119163 with aspirator of claimed structure
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A method is described for distributing samples within an automated analyzer from a linear arrangement of sample vessels to a processing plate in a two-dimensional n×m arrangement wherein samples are sorted, followed by transfer with a pipetting device with a linear arrangement to a processing vessel in a two-dimensional n×m arrangement and subsequent processing of samples using a second pipetting device which has a two-dimensional n×m arrangement.

Claims

1. A method for isolating analytes that may be present in fluid samples in an automated system, the method comprising the automated steps of:

a) loading samples on the automated system with identifiers, or identifying loaded samples according to sample type and analytical test to be performed;

b) transferring instructions from a control unit to a first and a second pipetting device comprising allocation of individual samples to a two-dimensional n×

m arrangement of individual tests in a processing plate;

c) transferring said fluid samples from at least two sample vessels to a at least two vessels of said processing plate with a first pipetting device comprising at least two pipetting units in linear arrangement, wherein said pipetting units are coupled to pipette tips;

d) combining together a solid support material and said fluid sample in a well of said processing plate for a period of time and under conditions sufficient to permit said analyte to be immobilized on the solid support material;

e) isolating the solid support material from other material present in the fluid sample in a separation station; and

f) purifying the analyte in the separation station by separating the fluid sample from the solid support material and washing the materials one or more times with a wash buffer, wherein said solid support material and said washing buffer are mixed by aspirating and dispensing with a second pipetting device, said second pipetting device comprising pipetting units in a two-dimensional n×

m arrangement for coupling to pipette tips;

wherein said pipette tips used in step c) are re-used in step f).

2. The method of claim 1, wherein said wells of said processing plate are integrally formed.

3. The method of claim 1, wherein said processing plate is a multiwell plate with a two-dimensional n×

m arrangement of vessels.

4. The method of claim 1, wherein said pipette tips are replaced in a tip rack in a two-dimensional n×

m arrangement following step c).

5. The method of claim 1, comprising the step of assigning any one pipette tip in said pipette tip rack to a specific vessel of said processing plate with a two-dimensional n×

m arrangement of vessels.

6. The method of claim 1, wherein the distance between two adjacent sample vessels is different from the distance between two adjacent vessels of the processing plate, and wherein the distance between two adjacent pipetting units of the first pipetting device is adjustable.

7. The method of claim 1, wherein in step b) instructions are transferred from said control unit to a first processor for transferring samples with said first pipetting device, and instructions are transferred from said control unit to a second processor for the steps carried out by said second pipetting device.