Method of measuring the affinity of biomolecules
First Claim
1. A method of measuring the affinity of a first and a second biomolecule, wherein the first biomolecule is tethered by a first tether portion having a first tether portion length and a first tether persistence length and the second biomolecule is tethered by a second tether portion having a second tether portion length and a second tether persistence length, wherein the first and second biomolecules are tethered to a surface, such that swept volumes defined by the movement of each biomolecule overlap, so that the first and second biomolecules are able to bind to each other, said method comprising determining a proportion of the first and second biomolecules bound to each other.
3 Assignments
0 Petitions
Accused Products
Abstract
The invention provides a method of measuring the affinity of first and second biomolecules in which a first biomolecule is tethered by a first tether portion having a first tether portion length and a second biomolecule is tethered by a second tether portion having a second tether portion length, the method comprising determining binding of adjacent first and second biomolecules to each other, varying at least one of the first and second tether lengths and determining binding of the first and second biomolecules. The invention also provides apparatus suitable for use in the method of the invention.
-
Citations
25 Claims
- 1. A method of measuring the affinity of a first and a second biomolecule, wherein the first biomolecule is tethered by a first tether portion having a first tether portion length and a first tether persistence length and the second biomolecule is tethered by a second tether portion having a second tether portion length and a second tether persistence length, wherein the first and second biomolecules are tethered to a surface, such that swept volumes defined by the movement of each biomolecule overlap, so that the first and second biomolecules are able to bind to each other, said method comprising determining a proportion of the first and second biomolecules bound to each other.
Specification