Microarray fabrication system and method
First Claim
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1. A method for preparing a biological microarray, comprising:
- providing an array of base pads at predetermined sites on a substrate, wherein individual base pads are configured to capture a nucleic acid molecule; and
contacting the array of base pads with a mixture of different nucleic acid molecules under conditions wherein a nucleic acid molecule is captured at each base pad,wherein a porous attachment layer is disposed over the base pads such that the porous attachment layer at least partially covers the base pads and the porous attachment layer is configured to attach amplified copies of the nucleic acid molecules comprising nucleotides or nucleotide-like components.
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Abstract
A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.
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Citations
18 Claims
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1. A method for preparing a biological microarray, comprising:
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providing an array of base pads at predetermined sites on a substrate, wherein individual base pads are configured to capture a nucleic acid molecule; and contacting the array of base pads with a mixture of different nucleic acid molecules under conditions wherein a nucleic acid molecule is captured at each base pad, wherein a porous attachment layer is disposed over the base pads such that the porous attachment layer at least partially covers the base pads and the porous attachment layer is configured to attach amplified copies of the nucleic acid molecules comprising nucleotides or nucleotide-like components. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method for preparing a biological microarray, comprising:
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forming an array of base pads at predetermined sites on a substrate; disposing a molecule binding substance over each of the base pads, thereby configuring each of the base pads to capture a single nucleic acid molecule by linking the single nucleic acid molecule to the molecule binding substance; and disposing a porous attachment layer over the base pads such that the base pads are in between the porous attachment layer and the substrate, wherein the porous attachment layer is configured to attach copies of the nucleic acid molecule, and wherein the copies of the nucleic acid molecule are obtained through amplifying the single nucleic acid molecule at each base pad.
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15. A method for preparing a biological microarray, comprising:
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forming a polymer layer on a substrate, wherein the polymer layer comprises a poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) polymer; disposing a photoresist layer over the polymer layer; forming interstitial spaces in the photoresist layer and the polymer layer; and removing the photoresist layer to expose polymer base pads, wherein the polymer base pads are coupled to a molecule binding substance.
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16. A method for preparing a biological microarray, comprising:
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forming a polymer layer on a surface of a substrate; forming a plurality of spaced apart photoresist regions on the polymer; contacting exposed portions of the polymer layer with a plurality of primers; and removing the photoresist regions and covered portions of the polymer layer such that a plurality of spaced apart polymer pads coupled to the plurality of primers remain on the surface of the substrate.
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17. A method for preparing a biological microarray, comprising:
providing an array of base pads at predetermined sites on a substrate, wherein individual base pads are configured to capture a nucleic acid molecule and a porous attachment layer disposed over the base pads, and wherein the base pads are formed by an imprint lithographic process, the imprint lithographic process comprising;
disposing a transfer layer on the substrate, disposing a light-curing resist layer over the transfer layer, patterning the resist layer via a mold, curing the patterned resist layer, etching the resist and transfer layers to expose regions of the substrate, depositing a pad-forming substance over the exposed regions of the substrate, and removing the resist and transfer layers.- View Dependent Claims (18)
Specification