Enzyme electrode

• US 8,784,624 B2
• Filed: 07/11/2007
• Issued: 07/22/2014
• Est. Priority Date: 07/12/2006
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Katsuki, Koji, Hirao, Konomu, Nagakawa, Kenji, Okamoto, Masashi, Fujinawa, Yoshiaki
• Primary Examiner(s): LEONG, SUSAN DANG
• Application Number:
  US12/309,222
• Publication Number:
  US 20100187107A1
• Time in Patent Office: 2,568 Days
• Field of Search: 20440301-40315, 205/777.5, 205/778, 205/792, 435 4- 4052, 422 681- 98, 436 62- 71, 436500-548
• US Class Current: 204/403.14
• CPC Class Codes: C12Q 1/001 Enzyme electrodes

Abstract

The present invention relates to an enzyme electrode including: a carbon particle; a metal particle held on the carbon particle, the metal particle having a catalytic activity against a redox reaction; a redox enzyme. The enzyme electrode of the present invention further includes a high-resistance particle enhancing an electrical resistance, the high-resistance particle being chemically stable. The high-resistance particle contains an inorganic substance, for example. The inorganic substance is aluminum oxide or smectite, for example.

Claims

1. An enzyme electrode comprising:

a carbon particle;

a metal particle held on the carbon particle, the metal particle having a catalytic activity against a redox reaction;

a redox enzyme; and

a high-resistance particle enhancing an electrical resistance, the high-resistance particle being chemically stable,

wherein the high-resistance particle creates electrical resistance of the enzyme electrode equal to or more than about 30 kΩ

, and

wherein the high-resistance particle has a particle size of 3 to 150 nm.

3. The enzyme electrode according to claim 1, wherein the high-resistance particle contains an inorganic substance.

4. The enzyme electrode according to claim 3, wherein the inorganic substance is aluminum oxide.

5. The enzyme electrode according to claim 3, wherein the inorganic substance is a hydrophilic fumed alumina.

6. The enzyme electrode according to claim 3, wherein the inorganic substance is silicate, or primarily contains the silicate.

7. The enzyme electrode according to claim 6, wherein the inorganic substance is a clay mineral.

8. The enzyme electrode according to claim 1, wherein the metal particle contains a noble metal.

9. The enzyme electrode according to claim 8, wherein the noble metal is at least one selected from the group comprising of platinum (Pt), rhodium (Rh), gold (Au), silver (Ag), palladium (Pd), ruthenium (Ru), iridium (Ir) and osmium (Os).

10. The enzyme electrode according to claim 9, wherein the noble metal is platinum (Pt).

11. The enzyme electrode according to claim 1, wherein the metal particle has a particle size of 1 to 4 nm.

12. The enzyme electrode according to claim 1, wherein the carbon particle has a particle size of 3 to 150 nm.

13. The enzyme electrode according to claim 1, wherein the redox enzyme is a glucose dehydrogenase.

14. The enzyme electrode according to claim 13, wherein the glucose dehydrogenase is a glucose dehydrogenase (FADGDH) containing flavin adenine dinucleotide (FAD) as a coenzyme.

15. The enzyme electrode according to claim 1, further comprising a binder for bonding the carbon particle.

16. The enzyme electrode according to claim 15, wherein the binder contains a petroleum wax or a fluorine sulfonic acid polymer.

17. The enzyme electrode according to claim 16, wherein the petroleum wax is at least one selected from the group comprising of a paraffin wax, a microcrystalline wax and petrolatum.

18. The enzyme electrode according to claim 17, wherein the petroleum wax is the paraffin wax.

19. The enzyme electrode according to claim 16, wherein the fluorine sulfonic acid polymer is a sulfonated tetrafluoroethylene-based polymer.

20. The enzyme electrode according to claim 7, wherein the clay mineral is smectite.

2. An enzyme electrode comprising:

a carbon particle;

a metal particle held on the carbon particle, the metal particle having a catalytic activity against a redox reaction;

a redox enzyme; and

a high-resistance particle enhancing an electrical resistance, the high-resistance particle being chemically stable,

wherein the high-resistance particle creates electrical resistance of the enzyme electrode equal to or more than about 30 kΩ

,

wherein the high-resistance particle contains an inorganic substance,

wherein the inorganic substance is silicate, or primarily contains the silicate,

wherein the inorganic substance is a clay mineral, and

wherein the clay mineral is smectite.