Immunodetection probe and method of immunodetection using the same

• US 8,785,136 B2
• Filed: 11/02/2010
• Issued: 07/22/2014
• Est. Priority Date: 12/09/2009
• Status: Active Grant

• Current Assignee: National Tsing Hua University
• Original Assignee: National Tsing Hua University
• Inventors: Tseng, Fan Gang, Tseng, Yuan Tai
• Primary Examiner(s): LAM, ANN Y
• Application Number:
  US12/917,706
• Publication Number:
  US 20110136132A1
• Time in Patent Office: 1,358 Days
• Field of Search: None
• US Class Current: 435/7.1
• CPC Class Codes: A61B 5/14528 invasively
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: A61B 5/6848 Needles

Abstract

An immunodetection probe comprises a needle structure having a compartment and configured to be inserted into an organic tissue, a dialysis membrane configured to isolate the compartment from the organic tissue, a detection device having a detection portion and a plurality of receptors, a first optical fiber coupled to the needle structure, and a pair of tubes connected to the compartment. The plurality of receptors are disposed on an end surface of the detection portion for conjugating target antibodies, wherein the detection portion is disposed in the compartment. The first optical fiber is configured to introduce light incident on photo-induced molecules adjacent to the end surface of the detection portion so as to cause a change in the pH level of the solution adjacent to the end surface of the detection portion. The pair of tubes is configured to transport the solution containing the photo-induced molecules into the compartment.

Claims

1. An immunodetection probe, comprising:

a needle structure configured to be inserted into an organic tissue, wherein the needle structure comprises a compartment;

a dialysis membrane configured to isolate the compartment from the organic tissue;

a detection device comprising a detection portion and a plurality of receptors, wherein the plurality of receptors are disposed on an end surface of the detection portion for conjugating target antibodies, and the detection portion is disposed in the compartment;

a first optical fiber coupled to the needle structure and configured to introduce light incident on photo-induced molecules adjacent to the end surface of the detection portion so as to cause a change in the pH level of solution adjacent to the end surface of the detection portion; and

a pair of tubes connected to the compartment, wherein one of the pair of tubes is configured to transport solution containing the photo-induced molecules into the compartment and another of the pair of the tubes is configured to transport the solution containing the photo-induced molecules out of the compartment.

2. The immunodetection probe of claim 1, further comprising a lens, wherein the lens is configured to constrain the irradiation range of the light from the first optical fiber, and the lens is disposed in the optical path of the light from the first optical fiber.

3. The immunodetection probe of claim 2, wherein the needle structure comprises a tip portion, and the tip portion comprises two opposite inclined surfaces, wherein the inclined surfaces are oppositely disposed on the end surface of the detection portion and disposed on an end surface of the first optical fiber, so as to reflect the light from the first fiber to the end surface of the detection portion.

4. The immunodetection probe of claim 3, wherein the lens is disposed on the end surface of the first optical fiber.

5. The immunodetection probe of claim 4, wherein the needle structure comprises a groove, the groove and the compartment are arranged in parallel, and the first optical fiber and the lens are disposed in the groove.

6. The immunodetection probe of claim 3, wherein the included angle between the opposite inclined surfaces of the tip portion is 90 degrees.

7. The immunodetection probe of claim 3, wherein the tip portion of the needle structure further comprises two metal layers, and the layers are respectively disposed on the inclined surfaces.

8. The immunodetection probe of claim 3, wherein the metal layers comprise silver.

9. The immunodetection probe of claim 1, wherein the detection portion of the detection device is disposed between the two tubes.

10. The immunodetection probe of claim 1, wherein the light comprises ultraviolet light.

11. The immunodetection probe of claim 1, wherein the receptor comprises an antibody.

12. The immunodetection probe of claim 1, wherein the photo-induced molecules comprise o-Nitrobenzaldehyde.

13. The immunodetection probe of claim 12, wherein the photo-induced molecules comprise PEG, and the o-Nitrobenzaldehyde molecules are contained within the PEG.

14. The immunodetection probe of claim 13, wherein the sizes of the photo-induced molecules range from 82 to 84 nm in diameter.

15. The immunodetection probe of claim 12, wherein the pH level is less than 3.5.

16. The immunodetection probe of claim 1, wherein the duration for which the light is incident on the photo-induced molecules adjacent to the end surface of the detection portion is one to ten minutes.

17. The immunodetection probe of claim 1, wherein the lens focuses the light adjacent to the end surface of the detection portion.

18. The immunodetection probe of claim 17, wherein the lens is configured to form a light spot 10 μ

m to 100 μ

m in diameter on the end surface of the detection portion.

19. The immunodetection probe of claim 17, wherein the focal length of the lens is 0.5 mm to 10 mm.

20. The immunodetection probe of claim 1, wherein the detection device is a fiber optic interferometer sensor.

21. The immunodetection probe of claim 1, wherein the first optical fiber is a single mode fiber or a multimode fiber.

22. The immunodetection probe of claim 1, wherein the detection portion comprises a second optical fiber, a first reflective layer, an intermediate layer, and a second reflective layer, the first and second reflective layers are disposed on opposite sides of the intermediate layer, and the first reflective layer is disposed on an end surface of the second optical fiber.

23. The immunodetection probe of claim 22, wherein the first and second reflective layers comprise gold.

24. The immunodetection probe of claim 22, wherein the intermediate layer comprises poly-dimethylsiloxane layer, and the length of the poly-dimethylsiloxane layer is 10 μ

m to 50 μ

m.