Neural stem cells
First Claim
Patent Images
1. A composition, comprising:
- neural stem cells;
EGF; and
FGF-2,wherein the neural stem cells are in a feeder-free, adherent monolayer culture and wherein at least 95% of said cells are symmetrically-dividing neural stem cells and wherein no more than 1% of the cells are positive for the expression of markers from mature astrocytes, neurons and oligodendrocytes.
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Abstract
A homogenous, symmetrically dividing population of adherent neural stem cells is obtained from ES cells or foetal or adult brain isolates, using an activator of a signalling pathway downstream of a receptor of the EGF receptor family, optionally in combination with an activator of a signalling pathway downstream of an FGF receptor. The neural stem cell population is highly pure and retains the ability to differentiate into neurons after in excess of 100 passages.
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Citations
12 Claims
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1. A composition, comprising:
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neural stem cells; EGF; and FGF-2, wherein the neural stem cells are in a feeder-free, adherent monolayer culture and wherein at least 95% of said cells are symmetrically-dividing neural stem cells and wherein no more than 1% of the cells are positive for the expression of markers from mature astrocytes, neurons and oligodendrocytes. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A mouse neural stem cell line, wherein said neural stem cells are maintained in the presence of EGF and FGF-2 in a feeder-free, adherent monolayer culture and wherein at least 95% of said cells are symmetrically-dividing neural stem cells and wherein no more than 1% of cells in the line are positive for expression of GFAP or β
- III tubulin.
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8. A human neural stem cell line, wherein said neural stem cells are maintained in the presence of EGF and FGF-2 in a feeder-free, adherent monolayer culture and wherein at least 95% of said cells are symmetrically-dividing neural stem cells and wherein no more than 1% of cells in the line are positive for expression of markers for mature astrocytes, neurons or oligodendrocytes.
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9. A population of neural stem cells, wherein said neural stem cells are maintained in the presence of EGF and FGF-2 in a feeder-free, adherent monolayer culture and wherein at least 95% of said cells are symmetrically-dividing neural stem cells and wherein no more than 1% of the cells are positive for the expression markers for mature astrocytes, neurons, and oligodendrocytes.
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10. A cell line, wherein the cells are maintained in the presence of EGF and FGF-2 in a feeder-free, adherent monolayer culture, wherein at least 95% of said cells are symmetrically-dividing neural stem cells and wherein no more than 1% of the cells are positive for the expression of markers for mature astrocytes, neurons, and oligodendrocytes.
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11. A composition comprising neural stem cells, EGF, and FGF-2, wherein the neural stem cells are in a feeder-free, adherent monolayer culture and at least 95% of the cells in the composition are symmetrically-dividing neural stem cells and wherein no more than 1% of the cells are positive for the expression of markers for mature astrocytes, neurons, and oligodendrocytes.
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12. A composition comprising neural stem cells, EGF, and FGF-2, wherein the neural stem cells are in a feeder-free, adherent monolayer culture, and wherein at least 95% of the cells in the composition are symmetrically-dividing neural stem cells.
Specification