Lipid therapy
First Claim
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1. A method for treating a subject comprising:
- withdrawing an amount of blood from the subject using a catheter assembly;
delipidating high density lipoprotein (HDL) present in the amount of blood, wherein delipidating comprises combining the amount of blood with a solvent and a surfactant;
adding a surface coating to the delipidated HDL for increasing a partition coefficient of the delipidated HDL; and
after delipidating, (1) combining an exogenous bioactive agent having a hydrophobic ligand bound thereto with the delipidated HDL with the surface coating, wherein the exogenous bioactive agent includes at least one of an apolipoprotein A1 (Apo A1), a functional Apo A1 mutant, and a functional Apo A1 mimetic peptide, and (2) forming a composition comprising (i) the delipidated HDL with the surface coating combined with the exogenous bioactive agent having a hydrophobic ligand bound thereto, and (ii) a viscosifier including at least one of hyaluronic acid, polyvinylpyrrolidone (PVP), a hydroxypropylmethacrylate (HPMA) copolymer system, and carboxymethyl cellulose (CMC); and
introducing the composition into a blood stream or tissue of the subject, using the catheter assembly.
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Abstract
A method including introducing into a blood stream a delipidated high density lipoprotein (HDL) and a bioactive agent. A composition including a delipidated high density lipoprotein (HDL) and an auxiliary agent in a form suitable for delivery into a blood vessel. A composition including Apo A1 comprising a hydrophobic ligand suitable to interact with cell surface binding sites. A composition including Apo A1 and an agent selected to one of increase the ATP-binding cassette protein 1 (ABCA1) transporter expression in macrophages and protect ABCA1 from thiol-mediated degradation.
15 Citations
18 Claims
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1. A method for treating a subject comprising:
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withdrawing an amount of blood from the subject using a catheter assembly; delipidating high density lipoprotein (HDL) present in the amount of blood, wherein delipidating comprises combining the amount of blood with a solvent and a surfactant; adding a surface coating to the delipidated HDL for increasing a partition coefficient of the delipidated HDL; and after delipidating, (1) combining an exogenous bioactive agent having a hydrophobic ligand bound thereto with the delipidated HDL with the surface coating, wherein the exogenous bioactive agent includes at least one of an apolipoprotein A1 (Apo A1), a functional Apo A1 mutant, and a functional Apo A1 mimetic peptide, and (2) forming a composition comprising (i) the delipidated HDL with the surface coating combined with the exogenous bioactive agent having a hydrophobic ligand bound thereto, and (ii) a viscosifier including at least one of hyaluronic acid, polyvinylpyrrolidone (PVP), a hydroxypropylmethacrylate (HPMA) copolymer system, and carboxymethyl cellulose (CMC); and introducing the composition into a blood stream or tissue of the subject, using the catheter assembly. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for treating a subject comprising:
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delivering to a treatment site within a lumen of a blood vessel of the subject, in a wall of the blood vessel of the subject, or periadvential area beyond the blood vessel by a percutaneous transluminal route, a composition comprising (i) a delipidated HDL and an exogenous bioactive agent having a hydrophobic ligand bound thereto, the delipidated HDL comprising an added surface coating having a property that increases a partition coefficient of the delipidated HDL into the treatment site, and (ii) a viscosifier, wherein the delivering step comprises locally delivering by placing a stent within the blood vessel of the subject, wherein the stent comprises the composition as a coating on a body of the stent or as a portion of the body of the stent, and wherein the viscosifier comprises at least one of hyaluronic acid, polyvinylpyrrolidone (PVP), a hydroxypropylmethacrylate (HPMA) copolymer system, and carboxymethyl cellulose (CMC), and wherein the exogenous bioactive agent includes at least one of an apolipoprotein A1 (Apo A1), a functional Apo A1 mutant, and a functional Apo A1 mimetic peptide. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18)
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Specification