Algal desaturases

• US 8,785,610 B2
• Filed: 05/06/2013
• Issued: 07/22/2014
• Est. Priority Date: 04/28/2011
• Status: Expired due to Fees

• Current Assignee: Aurora Algae, Incorporated (Reliance Industries Limited)
• Original Assignee: Aurora Algae, Incorporated (Reliance Industries Limited)
• Inventors: Kilian, Oliver, Vick, Bertrand
• Primary Examiner(s): Monshipouri, Maryam
• Application Number:
  US13/888,310
• Publication Number:
  US 20130281683A1
• Time in Patent Office: 442 Days
• Field of Search: 536/23.2, 435/320.1
• US Class Current: 536/23.2
• CPC Class Codes: C12N 9/0071 acting on paired donors with incorporation of molecular oxygen (1.14)
• CPC Class Codes: C12Y 114/19 with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)

Abstract

Provided herein are exemplary isolated nucleotide sequences encoding polypeptides having desaturase activity, which utilize fatty acids as substrates.

Claims

1. A transformation vector comprising an isolated nucleotide sequence encoding a polypeptide having desaturase activity, the nucleotide sequence set forth as SEQ ID NO:

1.

2. The transformation vector of claim 1 wherein the transformation vector encodes a functionally active desaturase which utilizes a fatty acid as a substrate.

3. The transformation vector of claim 2, wherein the functionally active desaturase comprises amino acids having the sequence set forth as SEQ ID NO:

6.

7. The transformation vector of claim 1 wherein the transformation vector is derived from algae.

8. The transformation vector of claim 7 wherein the algae is of genus Nannochloropsis.

4. A transformation vector comprising an isolated nucleotide sequence encoding a polypeptide having desaturase activity, the nucleotide sequence having at least 95% sequence identity to SEQ ID NO:

2.

5. The transformation vector sequence of claim 4 wherein the transformation vector encodes a functionally active desaturase which utilizes a fatty acid as a substrate.

6. The transformation vector of claim 5, wherein the functionally active desaturase comprises amino acids having the sequence set forth as SEQ ID NO:

7.