Fluorescence imaging module

• US 8,785,885 B1
• Filed: 01/30/2013
• Issued: 07/22/2014
• Est. Priority Date: 01/30/2013
• Status: Active Grant

• Current Assignee: Omnivision Technologies Incorporated (Will Semiconductor Co., Ltd. Shanghai)
• Original Assignee: Omnivision Technologies Incorporated (Will Semiconductor Co., Ltd. Shanghai)
• Inventors: Jutamulia, Suganda, Lei, Junzhao, Gadjali, Hasan
• Primary Examiner(s): TANINGCO, MARCUS H
• Application Number:
  US13/753,887
• Publication Number:
  US 20140209815A1
• Time in Patent Office: 538 Days
• Field of Search: 250/458.1
• US Class Current: 250/458.1
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16

Abstract

A fluorescence imaging module includes an image sensor and a lens disposed between a fluorescence sample and the image sensor to focus a fluorescence image of the fluorescence sample onto the image sensor. The fluorescence sample is to be positioned an object distance away from the lens. The lens is positioned an image distance away from the image sensor. The image distance is greater than the object distance. An illuminating device is disposed between the fluorescence sample and the lens. The illuminating device includes a light source and an optical element. The light source is adapted to emit light in a first direction towards the optical element. The optical element is optically coupled to receive the light and redirect the light in a second direction towards the fluorescence sample to illuminate the fluorescence sample.

Claims

1. A fluorescence imaging module, comprising:

an image sensor;

a lens disposed between a fluorescence sample and the image sensor to focus a fluorescence image of the fluorescence sample onto the image sensor, wherein the fluorescence sample is to be positioned an object distance away from the lens and wherein the lens is positioned an image distance away from the image sensor, wherein the image distance is greater than the object distance; and

an illuminating device disposed between the fluorescence sample and the lens, wherein the illuminating device includes a light source and an optical element, wherein the light source is adapted to emit light in a first direction towards the optical element and wherein the optical element is optically coupled to receive the light and redirect the light in a second direction towards the fluorescence sample to illuminate the fluorescence sample.

2. The fluorescence imaging module of claim 1 wherein the optical element includes a first surface optically coupled to receive the light in the first direction from the light source, wherein the optical element is adapted to refract the light to redirect the light in the second direction towards the fluorescence sample through a second surface of the optical element to illuminate the fluorescence sample.

3. The fluorescence imaging module of claim 1 wherein the light source is a first light source of a plurality of light sources included in the illuminating device, wherein each one of the plurality of light sources is adapted to emit light that is optically coupled to be received by the optical element, and wherein the optical element is adapted to refract the respective light received from each one of the plurality of light sources towards the fluorescence sample to illuminate the fluorescence sample.

4. The fluorescence imaging module of claim 3 wherein the optical element comprises a truncated pyramid disposed between the fluorescence sample and the lens, wherein each one of the plurality of light sources is adapted to emit light that is optically coupled to be received by a respective one of a plurality of side surfaces of the truncated pyramid, and wherein the truncated pyramid is adapted to refract the respective light received from each one of the plurality of light sources and redirect the respective light received from each one of the plurality of light sources towards the fluorescence sample through a surface of the truncated pyramid that faces the fluorescence sample to illuminate the fluorescence sample.

5. The fluorescence imaging module of claim 4 wherein the image sensor is adapted to acquire the fluorescence image of the fluorescence sample through the surface of the truncated pyramid that faces the fluorescence sample, through a surface of the truncated pyramid that faces the lens, and through the lens.

6. The fluorescence imaging module of claim 3 wherein the optical element comprises a truncated cone disposed between the fluorescence sample and the lens, wherein each one of the plurality of light sources is adapted to emit light that is optically coupled to be received by a side surface of the truncated cone, and wherein the truncated cone is adapted to refract the respective light received from each one of the plurality of light sources and redirect the respective light received from each one of the plurality of light sources towards the fluorescence sample through a surface of the truncated cone that faces the fluorescence sample to illuminate the fluorescence sample.

7. The fluorescence imaging module of claim 6 wherein the image sensor is adapted to acquire the fluorescence image of the fluorescence sample through the surface of the truncated cone that faces the fluorescence sample, through a surface of the truncated cone that faces the lens, and through the lens.

8. The fluorescence imaging module of claim 3 wherein the optical element comprises a plurality of prisms arranged between the fluorescence sample and the lens, wherein each one of the plurality of light sources is adapted to emit light that is optically coupled to be received by a first surface of a respective one of the plurality prisms, and wherein each respective one of the plurality of prisms is adapted to refract the light received from the respective one of the plurality of light sources and redirect the light received from the respective one of the plurality of light sources towards the fluorescence sample through a second surface of the respective one of the plurality of prisms to illuminate the fluorescence sample.

9. The fluorescence imaging module of claim 1 further comprising an emission filter disposed between the fluorescence sample and the lens to transmit light having an emission wavelength, wherein the image sensor is adapted to acquire the fluorescence image of the fluorescence sample through the lens and through the emission filter.

10. The fluorescence imaging module of claim 9, wherein the emission filter is removable.

11. The fluorescence imaging module of claim 9, wherein the emission filter is interchangeable.

12. The fluorescence imaging module of claim 1 further comprising:

a dichroic mirror disposed between the lens and the image sensor, wherein the dichroic minor is adapted to transmit light having an emission wavelength and reflect light having wavelengths other than the emission wavelength, wherein the image sensor is adapted to acquire the fluorescence image of the fluorescence sample through the lens and through the dichroic mirror; and

a second image sensor optically coupled to receive the light reflected from the dichroic minor having wavelengths other than the emission wavelength, wherein the second image sensor is adapted to acquire a non-fluorescence image of the fluorescence sample through the lens and from the dichroic minor.

13. The fluorescence imaging module of claim 12, wherein the dichroic mirror is interchangeable.

14. The fluorescence imaging module of claim 12 wherein a white light image of the fluorescence sample is obtained by summing the fluorescence image acquired by the image sensor and the non-fluorescence image acquired by the second image sensor.

15. The fluorescence imaging module of claim 1 wherein the light source comprises one of an ultraviolet LED, a blue LED, a green LED, an ultraviolet laser diode, a blue laser diode, and a green laser diode.

16. The fluorescence imaging module of claim 3 wherein the plurality of light source comprise at least one of a white LED, an ultraviolet LED, a blue LED, a green LED, an ultraviolet laser diode, a blue laser diode, and a green laser diode.

17. The fluorescence imaging module of claim 1 wherein the lens comprises an auto-focused lens.

18. The fluorescence imaging module of claim 1 wherein the lens is included in a lens system.

19. The fluorescence imaging module of claim 1 wherein a magnification of the fluorescence image of the fluorescence sample is substantially equal to a ratio of the image distance to the object distance.

20. A fluorescence imaging module, comprising:

an image sensor;

a lens disposed between a fluorescence sample and the image sensor to focus a fluorescence image of the fluorescence sample onto the image sensor, wherein the fluorescence sample is to be positioned an object distance away from the lens and wherein the lens is positioned an image distance away from the image sensor, wherein a total distance of the fluorescence sample to the image sensor is substantially equal to a sum of the image distance and the object distance; and

an illuminating device disposed between the fluorescence sample and the lens, wherein the illuminating device includes a light source and an optical element, wherein the light source is adapted to emit light in a first direction towards the optical element and wherein the optical element is optically coupled to receive the light and redirect the light in a second direction towards the fluorescence sample to illuminate the fluorescence sample.

21. The fluorescence imaging module of claim 20 wherein the optical element includes a first surface optically coupled to receive the light in the first direction from the light source, wherein the optical element is adapted to refract the light to redirect the light in the second direction towards the fluorescence sample through a second surface of the optical element to illuminate the fluorescence sample.

22. The fluorescence imaging module of claim 20 wherein the light source is a first light source of a plurality of light sources included in the illuminating device, wherein each one of the plurality of light sources is adapted to emit light that is optically coupled to be received by the optical element, and wherein the optical element is adapted to refract the respective light received from each one of the plurality of light sources towards the fluorescence sample to illuminate the fluorescence sample.

23. The fluorescence imaging module of claim 22 wherein the optical element comprises a truncated pyramid disposed between the fluorescence sample and the lens, wherein each one of the plurality of light sources is adapted to emit light that is optically coupled to be received by a respective one of a plurality of side surfaces of the truncated pyramid, and wherein the truncated pyramid is adapted to refract the respective light received from each one of the plurality of light sources and redirect the respective light received from each one of the plurality of light sources towards the fluorescence sample through a surface of the truncated pyramid that faces the fluorescence sample to illuminate the fluorescence sample.

24. The fluorescence imaging module of claim 22 wherein the optical element comprises a truncated cone disposed between the fluorescence sample and the lens, wherein each one of the plurality of light sources is adapted to emit light that is optically coupled to be received by a side surface of the truncated cone, and wherein the truncated cone is adapted to refract the respective light received from each one of the plurality of light sources and redirect the respective light received from each one of the plurality of light sources towards the fluorescence sample through a surface of the truncated cone that faces the fluorescence sample to illuminate the fluorescence sample.

25. The fluorescence imaging module of claim 22 wherein the optical element comprises a plurality of prisms arranged between the fluorescence sample and the lens, wherein each one of the plurality of light sources is adapted to emit light that is optically coupled to be received by a first surface of a respective one of the plurality prisms, and wherein each respective one of the plurality of prisms is adapted to refract the light received from the respective one of the plurality of light sources and redirect the light received from the respective one of the plurality of light sources towards the fluorescence sample through a second surface of the respective one of the plurality of prisms to illuminate the fluorescence sample.

26. The fluorescence imaging module of claim 20 wherein a magnification of the fluorescence image of the fluorescence sample is substantially equal to the ratio of the image distance to the object distance.