Dispenser with filter device

• US 8,790,596 B2
• Filed: 05/31/2012
• Issued: 07/29/2014
• Est. Priority Date: 06/01/2011
• Status: Active Grant

• Current Assignee: Ventana Medical Systems Incorporated (Roche Holding AG)
• Original Assignee: Ventana Medical Systems Incorporated (Roche Holding AG)
• Inventors: Roper, Philip G., Talucci, Kevin, Rowland, Shane
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): FISHER, BRITTANY I
• Application Number:
  US13/485,798
• Publication Number:
  US 20120308445A1
• Time in Patent Office: 789 Days
• Field of Search: 422/513
• US Class Current: 422/513
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 2035/1023 using a valve in the tip or nozzle
• CPC Class Codes: G01N 2035/1053 for separating part of the liquid, e.g. filters, extraction phase
• CPC Class Codes: G01N 35/1016 Control of the volume dispensed or introduced

Abstract

A biological processing system includes a platform assembly for holding slides and a dispenser assembly with dispensers. The dispensers are sequentially positionable over specimen-bearing slides to enable dispensing of substances onto specimens. The dispensers include a filter device for filtering a processing substance such that a substantially precipitate-free filtrate is applied to the specimens. The dispenser includes a filter device positioned at least partially in a reservoir chamber. The filter device includes anti-clogging features to maintain desired performance.

Claims

1. A fluid dispenser for a biological sample processing system, the fluid dispenser comprising:

a barrel including a main body and a piston coupled to the main body, the main body including a sidewall and a bottom that at least partially define a reservoir chamber for holding fluid;

a valve positioned downstream of the reservoir chamber; and

a filter device upstream of the valve and including a protective cantilevered member and a vertically oriented filtering element having a plurality of through-holes with inlets positioned closer to a longitudinal axis of the filter device than an outer periphery of protective cantilevered member positioned above the plurality of through-holes, wherein the filtering element extends upwardly through at least a portion of the reservoir chamber when the barrel is in a vertical orientation to allow the fluid, which is in the reservoir chamber and located between the filtering element and the sidewall of the main body, to pass laterally through the filtering element and to flow towards the valve while the filtering element substantially blocks precipitates in the fluid having a threshold size.

2. The fluid dispenser of claim 1, wherein the valve is positioned in the barrel and another valve is positioned downstream of the barrel, the filtering element configured to block precipitates sufficiently large to cause malfunctioning of at least one of the valve positioned in the barrel and the valve positioned downstream of the barrel.

3. The fluid dispenser of claim 1, wherein the filter device includes at least one anti-clogging element configured to keep precipitates, which are in the reservoir chamber, away from the filtering element of the filter device.

4. The fluid dispenser of claim 3, wherein the filter device includes a hollow main body with a flow lumen and an outlet port, and wherein the at least one anti-clogging element includes a particle blocking member that extends outwardly from the hollow main body beyond the filtering element of the filter device.

5. The fluid dispenser of claim 1, wherein the filter device includes a protrusion that extends towards the sidewall of the main body so as to obstruct precipitates moving vertically through the fluid generally toward the inlets.

6. The fluid dispenser of claim 1, wherein the filter device includes a collector rib extending laterally outward from a main body of the filtering element, the collector rib configured to block particles below the collector rib from moving upwardly towards the filtering element.

7. The fluid dispenser of claim 1, wherein the filter device is positioned at a bottom of the reservoir chamber, the filter device further comprises a main body that extends upwardly partially through the reservoir chamber, and the filtering element is positioned along a sidewall of the main body of the filter device.

8. The fluid dispenser of claim 1, wherein the filtering element is configured to substantially block precipitates having outer diameters equal to about 0.01 inch.

9. The fluid dispenser of claim 1, wherein the filtering element includes a pair of spaced apart perforated sidewalls that allow fluid flow into and through an interior region of the filtering device.

10. The fluid dispenser of claim 1, wherein the filtering element includes a vertical sidewall through which the plurality of through-holes extends, wherein the vertical sidewall is spaced apart from the sidewall of the main body such that fluid directly between the vertical sidewall of the filtering element and the sidewall of the main body flows horizontally through the through-holes.

11. A biological processing system, comprising:

a platform assembly including a plurality of slide holders; and

a dispenser assembly including a plurality of dispensers, the platform assembly and the dispenser assembly configured to cooperate to sequentially position the dispensers relative to specimen-bearing slides on the slide holders to enable dispensing of substances onto the specimen-bearing slides, wherein at least one of the dispensers includes a filter device for filtering a substance to deliver a substantially precipitate-free filtrate through a valve and onto one of the specimen-bearing slides, the filter device including

a protective cantilevered member, and

a vertically oriented sidewall including plurality of through-holes with inlets positioned closer to a longitudinal axis of the filter device than an outer periphery of the protective cantilevered member positioned above or below the plurality of through-holes.

12. The biological processing system of claim 11, wherein the at least one dispenser comprises:

a barrel holder including a biasing member and an outlet; and

a barrel guided within the barrel holder, the barrel including a main body and a piston coupled to the main body, the main body defining a reservoir chamber for holding a fluid, wherein the sidewall is perforated to allow fluids in the reservoir chamber to pass therethrough while substantially blocking precipitates of a threshold size from exiting the barrel.

13. The biological processing system of claim 11, wherein the plurality of dispensers contain fluids for performing at least one staining protocol, and the at least one dispenser contains a DAB reagent.

14. The biological processing system of claim 11, wherein the sidewall is configured to substantially block precipitates in a chamber of the at least one dispenser while allowing the substance to flow into an interior chamber of the filter device.

15. The biological processing system of claim 11, wherein the sidewall extends upwardly through at least a portion of a reservoir chamber of the at least one dispenser to allow the substance in the reservoir chamber to flow horizontally through the through-holes and to flow vertically through the valve.

16. A fluid dispenser for a biological sample processing system, the fluid dispenser comprising:

a barrel including a main body and a piston coupled to the main body, the main body including a sidewall and a bottom at least partially defining a reservoir chamber for holding fluid;

a valve; and

a filter device positioned upstream of the valve and including a vertically oriented filtering element having a plurality of through-holes, wherein the filtering element extends upwardly through at least a portion of the reservoir chamber and is spaced apart from the sidewall of the main body such that fluid located directly between the filtering element and the sidewall of the main body flows laterally through the through-holes when the barrel is in a generally vertical orientation.

17. The fluid dispenser of claim 16, wherein the filter device includes at least one anti-clogging element positioned beneath the filtering element, wherein the anti-clogging element is configured to keep precipitates, which are at a bottom of the reservoir chamber, from traveling upwardly towards the filtering element.

18. The fluid dispenser of claim 17, wherein the at least one anti-clogging element includes a collector rib that extends laterally outward from a main body of the filter device.

19. The fluid dispenser of claim 16, wherein the filter device includes a protective cantilevered member extending laterally outward from a main body of the filter device such that the protective cantilevered member blocks particles, which are located in the reservoir chamber, from moving vertically towards the filtering element.

20. The fluid dispenser of claim 16, wherein the filter device further comprises:

an anti-clogging element that extends laterally outward beyond the filtering element such that the anti-clogging element is located above the bottom of the barrel to block particles in fluid traveling upward away from the bottom of the barrel; and

a protrusion that extends laterally outward beyond the filtering element and towards a sidewall of the barrel so as to obstruct precipitates moving downward through the fluid generally toward the inlets.

21. The fluid dispenser of claim 16, wherein the filtering element is a first filtering element, and wherein the filter device further comprises

a second filtering element, and

an interior chamber, wherein the second filter element extends vertically through at least a portion of the reservoir chamber, and wherein the interior chamber is located between the first and second filtering elements.

22. The fluid dispenser of claim 21, wherein each of the first and second filtering elements is a perforated vertical wall.

23. The fluid dispenser of claim 16, wherein the filtering device includes an upper particle blocking member and a lower particle blocking member, the upper particle blocking member and the lower particle blocking member protruding outwardly from the main body to define a substantially horizontal flow channel through which fluid is capable of flowing to access the at least one filtering element.