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Methods of amplifying and sequencing nucleic acids

  • US 8,790,876 B2
  • Filed: 04/20/2007
  • Issued: 07/29/2014
  • Est. Priority Date: 01/29/2003
  • Status: Active Grant
First Claim
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1. A method for sequencing nucleic acids comprising:

  • (a) delivering the nucleic acids to an array of at least 10,000 reaction chambers on a planar -surface, such that a plurality of the reaction chambers comprise beads capable of binding the nucleic acid and no more than one nucleic acid;

    (b) amplifying the nucleic acids in the reaction chambers, wherein a plurality of amplified copies of the nucleic acids are immobilized to beads; and

    (c) performing a sequencing reaction simultaneously in a plurality of the reaction chambers comprising the immobilized amplified copies of the nucleic acids.

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