Methods of amplifying and sequencing nucleic acids
First Claim
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1. A method for sequencing nucleic acids comprising:
- (a) delivering the nucleic acids to an array of at least 10,000 reaction chambers on a planar -surface, such that a plurality of the reaction chambers comprise beads capable of binding the nucleic acid and no more than one nucleic acid;
(b) amplifying the nucleic acids in the reaction chambers, wherein a plurality of amplified copies of the nucleic acids are immobilized to beads; and
(c) performing a sequencing reaction simultaneously in a plurality of the reaction chambers comprising the immobilized amplified copies of the nucleic acids.
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Abstract
An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
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Citations
7 Claims
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1. A method for sequencing nucleic acids comprising:
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(a) delivering the nucleic acids to an array of at least 10,000 reaction chambers on a planar -surface, such that a plurality of the reaction chambers comprise beads capable of binding the nucleic acid and no more than one nucleic acid; (b) amplifying the nucleic acids in the reaction chambers, wherein a plurality of amplified copies of the nucleic acids are immobilized to beads; and (c) performing a sequencing reaction simultaneously in a plurality of the reaction chambers comprising the immobilized amplified copies of the nucleic acids. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification